A112030

DMT-dA(tac) Phosphoramidite

configured for ABI

• Synonym(s): DMT-dA(tac) amidite
• Empirical Formula (Hill Notation): C₅₂H₆₂N₇O₈P
• CAS Number: 149989-58-2
• Molecular Weight: 944.06
• MDL number: MFCD00058443
• UNSPSC Code: 12352200
• PubChem Substance ID: 329770672
• NACRES: NA.51

Properties

• type: for DNA synthesis
• product line: Proligo Reagents
• Assay: ≥98% (³¹P-NMR); ≥98.0% (reversed phase HPLC)
• form: powder
• technique(s): oligo synthesis: suitable
• λ: conforms (UV/VIS Identity)
• compatibility: configured for ABI
• nucleoside profile: base: deoxyadenosine; base protecting group: TAC; 2' protecting group: none; 5' protecting group: DMT; deprotection: fast
• storage temp.: -10 to -25°C
• SMILES string: COc1ccc(cc1)C(OC[C@H]2O[C@H](C[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)n3cnc4c(NC(=O)COc5ccccc5)ncnc34)(c6ccccc6)c7ccc(OC)cc7
• InChI: 1S/C48H54N7O8P/c1-33(2)55(34(3)4)64(61-27-13-26-49)63-41-28-44(54-32-52-45-46(50-31-51-47(45)54)53-43(56)30-59-40-16-11-8-12-17-40)62-42(41)29-60-48(35-14-9-7-10-15-35,36-18-22-38(57-5)23-19-36)37-20-24-39(58-6)25-21-37/h7-12,14-25,31-34,41-42,44H,13,27-30H2,1-6H3,(H,50,51,53,56)/t41-,42+,44+,64?/m0/s1
• InChI key: INUFZOANRLMUCW-SDNJGBRXSA-N

Description

General description

Proligo′s DNA phosphoramidites lead to high-yield and high-quality oligonucleotides due to their high coupling efficiency. The deprotection step of automated oligonucleotide synthesis is integral to synthesis time and final product quality. The high coupling efficiency of Proligo′s DNA phosphoramidites leads to high-yield and high-quality oligonucleotides. Substitution of standard protecting groups with the labile TAC (tert.-butylphenoxyacetyl) protecting group results in ultra-fast and easy deprotection under mild conditions, suitable for oligonucleotides with base labile monomers and reporters as well as in-situ synthesis schemes on glass surfaces. Key Features of TAC Chemistry:

• Deprotection of the TAC group is ultra-fast: complete deprotection in concentrated ammonia occurs within 15 minutes at 55 °C or two hours atroom temperature
• Compatible with the AMA deprotection reagent (a mixture of ≥25%ammonia in water with 40% aqueous methylamine I/I, v/v)
• Highly soluble in acetonitrile. No need to add co-solvents such as dimethylformamide or methylene chloride
• Suitable for the synthesis of oligomers with base-labile units e.g., dyes and modifiers, because of less exposure to ammonia and the possibility ofroom temperature deprotection
• No change is required in the reagents commonly used for DNA synthesis,except that Proligo′s Fast Deprotection Cap A solution is used instead ofCap A solution
• The application of dA(tac) minimizes depurination and improves thequality of oligonucleotides

DMT-dA(tac) Phosphoramidite configured for ABI Synthesizers.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable