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14571C

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EX-CELL® 293 Serum-Free Medium for HEK 293 Cells

Serum-free, Animal-protein free, without L-glutamine, liquid, suitable for cell culture

Pharma Manufacturing

Synonym(s):

HEK 293 Medium

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About This Item

MDL number:
MFCD01634638
UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

500

sterility

sterile; sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable (suspension)

components

NaHCO3: yes
HEPES: no
L-glutamine: no
glucose: yes

shipped in

ambient

storage temp.

2-8°C

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Application

EX-CELL 293 is an animal-protein free, serum-free medium developed for the long-term growth of Human Embryonic Kidney 293 (HEK 293) and related cells. The cells, in a suspension culture, can be subcultured directly into EX-CELL 293 from serum-supplemented media with little or no adaptation. Suspension cultures can be maintained, without refeeding, for about 10 days and can be carried for more that 20 passages with no loss of viability.
EX-CELL 293 is an animal-protein free, serum-free medium developed for the long-term growth of Human Embryonic Kidney 293 (HEK 293) and related cells. The cells, in a suspension culture, can be subcultured directly into EX-CELL 293 from serum-supplemented media with little or no adaptation. Suspension cultures can be maintained, without refeeding, for about 10 days and can be carried for more that 20 passages with no loss of viability.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jan Bláha et al.
Protein expression and purification, 140, 36-43 (2017-08-02)
Human natural killer receptor protein 1 (NKR-P1, CD161, gene klrb1) is a C-type lectin-like receptor of natural killer (NK) cells responsible for recognition of its cognate protein ligand lectin-like transcript 1 (LLT1). NKR-P1 is the single human orthologue of the
Jan Bláha et al.
Protein expression and purification, 109, 7-13 (2015-01-28)
Lectin-like transcript 1 (LLT1, gene clec2d) was identified to be a ligand for the single human NKR-P1 receptor present on NK and NK-T lymphocytes. Naturally, LLT1 is expressed on the surface of NK cells, stimulating IFN-γ production, and is up-regulated
G Schoofs et al.
Cytotechnology, 28(1-3), 81-89 (2008-11-13)
We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum
Pratik Jaluria et al.
Biotechnology and bioengineering, 99(6), 1443-1452 (2007-11-21)
Animal-derived serum is an essential media supplement for mammalian cells in cell culture. For a number of reasons including cost, regulatory concerns, lot inconsistency, potential contamination with adventitious agents, and down-stream processing it is desirable to eliminate the use of
J B Kaufman et al.
Cytotechnology, 33(1-3), 3-11 (2008-11-13)
The extracellular domain of human parathyroid Ca(2+) receptor was needed in order to study itsstructure and clinical application. The Ca(2+)receptor is a unique member of the G protein-coupledreceptor super-family, expressed in parathyroid andkidney cells where it has been shown to

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