89747

Tinsdale Agar Base

NutriSelect^® Plus, suitable for microbiology

• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15 g/L ; L-cystine, 0.24 g/L ; peptic digest of animal tissue, 20 g/L ; sodium chloride, 5 g/L ; sodium thiosulfate, 0.43 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• application(s): clinical testing; environmental; food and beverages; veterinary; microbiology
• suitability: selective and differential for Corynebacterium spp.

Description

Application

Tinsdale Agar is used for the primary isolation and identification of Corynebacterium diptheriae.
The medium differentiates between C. diphtheriae and the diphtheroids found in the upper respiratory tract. This differentiation was based on the ability of C. diphtheriae to produce grayish-black colonies, surrounded by a brown/black halo. Diphtheroids do not have this ability. The dark halo is due to the production of H₂S from cystine and sodium thiosulfate, interacting with the tellurite salt.

Preparation Note

Suspend 40.7g of agar base in 1000ml of distilled water. Bring to the boil and dissolve completely. DO NOT AUTOCLAVE. Allow to cool to 50°C and add aseptically the contents of 1 unit (part A+B) of Tinsdale Supplement (Cat. No. 73831). Mix throughly and pour into sterile dishes.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves