85627

SPS Agar

suitable for microbiology, NutriSelect^® Basic

• Synonym(s): Perfringens Selective Agar acc. to Angelotti
• UNSPSC Code: 41171609
• NACRES: NA.74

Properties

• sterility: non-sterile
• Quality Level: 100
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 13.9 g/L ; casein peptone, 15 g/L ; ferric citrate, 0.5 g/L ; polymyxin B sulfate, 0.01 g/L ; sodium sulfadiazine, 0.12 g/L ; sodium sulfite, 0.5 g/L ; yeast extract, 10 g/L
• manufacturer/tradename: NutriSelect^® Basic
• technique(s): microbiological culture: suitable
• final pH: 7.0±0.2 (25 °C)
• application(s): bioburden testing; environmental; food and beverages; microbiology
• suitability: selective and differential for Clostridium spp.

Description

Application

SPS Agar (Sulfite Polymyxin Sulfadizine Agar) is a solid medium for detection, isolation and enumeration of Clostridium perfringens and Clostridium botulinum in food acc. to Angelotti et al. (1962).

Sulfite Polymyxin Sulfadizine Agar (SPS) may be used in the detection of V. cholerae and V. vulnificus in the environment.

Preparation Note

Dissolve 40 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves