11336045001

DNA Molecular Weight Marker VIII

pkg of 50 μg (in 200 μl), solution

• Synonym(s): DNA marker
• UNSPSC Code: 41105335

Properties

• form: solution
• Quality Level: 100
• packaging: pkg of 50 μg (in 200 μl)
• manufacturer/tradename: Roche
• concentration: 250 μg/mL
• color: colorless
• solubility: water: miscible
• storage temp.: −20°C

Description

General description

DNA Molecular Weight Marker VIII contains a mixture of 17 DNA fragments.
The product is also available as DNA Molecular Weight Marker VIII, Digoxigenin-labeled.
Size range: 19 to 1114 bp

Application

DNA Molecular Weight Marker VIII is used for the size determination of DNA in agarose gels by:

• Restriction digests
• PCR
• RT-PCR

Note: Use this marker to size DNA fragments between 19 and 1,114 bp.
This marker is also available in DIG-labeled form (DNA Molecular Weight Marker VIII, DIG-labeled).

Features and Benefits

Fragment mixture prepared by cleavage of pUCBM21 DNA with Hpa II, and pUCBM21 DNA with Dra I and Hind III.
Contents
Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0).

Sequence

The mixture contains 17 DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 19, 26, 34/34, 37, 67, 110, 124, 147, 190, 242, 320, 404, 489, 501, 692, 900, and 1114 bp.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

Unit Definition

50 μg = 1A₂₆₀ unit

Preparation Note

Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Use this marker to size DNA fragments between 19 and 1,114 bp.
This marker is also available in DIG-labeled form (DNA Molecular Weight Marker VIII, DIG-labeled).

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): does not flash
• Flash Point(C): does not flash