SCT271

BioTracker^™ Green Endocytosis Live Cell Probe

Human

• Synonym(s): endocytosis fluorescent probe, endocytosis live cell probe, endocytosis probe, green endocytosis probe
• UNSPSC Code: 41106514
• NACRES: NB.12

Properties

• product name: BioTracker^™ Green Endocytosis Live Cell Probe,
• biological source: human
• Quality Level: 100
• form: lyophilized
• packaging: vial of 40 μL
• manufacturer/tradename: Millipore
• technique(s): cell culture | stem cell: suitable
• shipped in: dry ice
• storage temp.: -10 to -25°C

Description

General description

BioTracker^™ Green Endocytosis Live Cell Probe is a pH-dependent fluorescent dye that localizes to the vesicle membrane. Visualization of endocytosis using SCT271 is a more direct method than fluorescent analogs, and allows visualization of endocytosis.

*Precise visualization of endocytosis
*Track endocytosis using live cells
*High responsiveness to pH change


SPECTRAL PROPERTIES
λex: 386 nm
λem: 522 nm

Suggested observation conditions:
λex: 350 - 410 nm
λem: 500 - 560 nm

Features and Benefits

pH-dependent fluorescent live cell probe for visualization of endocytosis from the early endosome stage.
Fluorescent Dye-Dextran Conjugates or membrane staining reagents are used to visualize endocytosis. However, they have limitations in observing the dynamics of endosomes in live cells in terms of precision of staining or retentivity of reagent. SCT271 is a different kind of reagent that overcomes these limitations.

Target description

Endocytosis is a cellular process that describes macromolecules being taken up through the plasma membrane-derived endosome vesicle. The endocytic pathway contributes to maintaining intracellular homeostasis by transporting various nutrients into the cell and shuttling unneeded components to the lysosome, which functions as a waste disposal system. It has been demonstrated that disruption of endocytosis is related to some neurodegenerative dysfunction and immune diseases. Investigation of the endocytic pathway is therefore of increasing interest to cell biologists and in diverse disease disciplines.

Physical form

green liquid

Storage and Stability

Store at -20 °C, protected from light.

Other Notes

This live cell probe is a non membrane-permeable molecule that fluoresces on internalization into mammalian cells,enabling direct observation of the membrane of internalized vesicles.

Legal Information

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable