MABN2300

Anti-PSMA/FOLH1 Antibody, clone 3F11

• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies, primary antibodies
• clone: 3F11, monoclonal
• mol wt: calculated mol wt 84.33 kDa; observed mol wt ~120 kDa
• purified by: using protein G
• species reactivity: mouse, human, rat, canine, porcine
• packaging: antibody small pack of 100 μg
• technique(s): immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: Extracellular domain
• UniProt accession no.: Q04609
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... FOLH1(2346)

Description

General description

Glutamate carboxypeptidase 2 (UniProt: Q04609; also known as EC:3.4.17.21, Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, FGCP, Glutamate carboxypeptidase II, GCPII, Membrane glutamate carboxypeptidase, mGCP, N-acetylated-alpha-linked acidic dipeptidase I, NAALADase I, Prostate-specific membrane antigen, PSM, PSMA, Pteroylpoly-gamma-glutamate carboxypeptidase) is encoded by the FOLH1 (also known as FOLH, NAALADI, PSM, PSMA, GIG27) gene (Gene ID: 2346) in human. PSMA is a single-pass type II membrane protein that has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase activities. It exhibits a preference for tri-alpha-glutamate peptides. Its expression pattern is restricted mainly to the healthy prostate secretory-acinar epithelium and the plasma membrane of epithelial prostate carcinoma. In the brain, it modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG) and release of glutamate. Clone 3F11 is shown to be highly specific for PSMA and does not exhibit any cross-reactivity with unrelated proteins. It binds to a linear epitope (aa 226-243) within the extracellular domain and is reported to be suitable for the detection of PSMA orthologs from some other species when denatured form of PSMA is used. (Ref.: Vahidfar, N., et al., (2019). J. Radioanal. Nucl. Chem. 322:237 248; Novakova, Z., et al. (2017). The Prostate 77(7); 749-764).

Specificity

Clone 3F11 is a mouse monoclonal antibody that detects Prostate-specific membrane antigen (PSMA). It targets an epitope within 18 amino acids from the extracellular domain.

Immunogen

Full-length recombinant human Prostate-specific membrane antigen (PSMA).

Application

Quality Control Testing

Evaluated by Western Blotting in LNCap cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PSMA in LNCap cell lysate.

Tested Applications

Immunofluorescence Analysis: A representative lot detected PSMA in Immunofluorescence applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).

ELISA Analysis: A representative lot detected PSMA in ELISA applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).

Immunohistochemistry (Paraffin) Analysis: A 1:80,000 dilution from a representative lot detected PSMA in human prostate tissues (Courtesy of Barinka, Institute of Biotechnology of the Czech Academy of Sciences, Vestec, Czech Republic and J. Hrudka, University Hospital Vinohrady, Prague, Czech Republic).

Immunohistochemistry Applications: A representative lot detected PSMA in Immunohistochemistry applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).

Immunocytochemistry Analysis: A representative lot detected PSMA in Immunocytochemistry applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).

Western Blotting Analysis: A representative lot detected PSMA in Western Blotting applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-PSMA/FOLH1, clone 3F11, Cat. No. MABN2300, is a mouse monoclonal antibody that detects Prostate-specific membrane antigen (PSMA) and is tested in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1