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IEVH85R

Millipore

Immobilon® -E PVDF Membrane

1 roll, 8.5 cm × 10 m, 0.45 µm pore size, transfer membrane wets out in transfer buffer or water

Synonym(s):

Immobilon® NOW, Immobilon® narrow roll, Western blotting membrane, blotting membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031613
NACRES:
NB.23

product name

Immobilon®-E PVDF Membrane, The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.

material

PVDF membrane
plain filter
white filter

Quality Level

400

feature

easily wetted with water

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

filter L × W

8.5 cm × 10 m

pore size

0.45 μm pore size

compatibility

for use with Amido black
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Transillumination

detection method

chemiluminescent
colorimetric
radioactive

shipped in

ambient

Related Categories

General description

Filter Code: IEVH
Immobilon-E is the first PVDF membrane for Western blotting that can be wetted with with aqueous transfer buffer. This eliminates the alcohol pre-wet step required with other PVDF membranes.
Primary Binding Mechanisms: Electrostatic, hydrophobic
This 8.5cm x 10m roll offers 1-cut convenience for standard mini-blots (7 x 8.5cm) and midi-blots (8.5 x 13.5cm). It works with the optional Immobilon NOW Dispenser (catalog number IMDISP).

Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most Western blotting applications, especially proteins greater than 20 kDa
- Compatible with chemiluminescent and chromogenic detection

Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes.

Features & Benefits:
- High protein binding provides stronger signal
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be stripped and reprobed multiple times

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

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Articles

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

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