CBL610
Mouse IgG1 Negative Control Antibody, clone 1E2.2
clone 1E2.2, 1 mg/mL, Chemicon®
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.42
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
clone
1E2.2, monoclonal
manufacturer/tradename
Chemicon®
concentration
1 mg/mL
technique(s)
flow cytometry: suitable
isotype
IgG1κ
shipped in
wet ice
target post-translational modification
unmodified
General description
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Specificity
This isotype control shows negligible cross-reactivity with human cell surface antigens on tissue sections or in cellular preparations. It therefore enables the estimation of non-specific binding of mouse monoclonals to cell surface antigens.
Application
Mouse IgG1 Negative Control Monoclonal Antibody validated for use in Flow Cytometry.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Epitope Tags
Epitope Tags
Quality
Evaluated by Flow Cytometry in HeLa cells.
Flow Cytometry Analysis: 2 µg of this antibody was used as a Mouse IgG1 Negative Control in staining of HeLa cells.
Flow Cytometry Analysis: 2 µg of this antibody was used as a Mouse IgG1 Negative Control in staining of HeLa cells.
Linkage
Replaces: CBL600
Physical form
Format: Purified
Protein G
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cells
HeLa cells
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Minimum order quantity required to qualify. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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