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biological source
human
Quality Level
100
300
Assay
>98%
form
liquid
manufacturer/tradename
Chemicon®
NCBI accession no.
UniProt accession no.
General description
High Density Lipoprotein (HDL) isolated by ultracentrifugation are delipidated. Apo A-I is purified by column chromatography in 6M urea (Schonfeld et al. 1977). Purity is determined by quantitative densitometric of SDS polyacrylamide gels (Laemlli 1970). Protein is determined by the Pierce BCA method (which was found to be comparable to the Lowry method) with BSA as the standard.
MOLECULAR WEIGHT:
28,000
MOLECULAR WEIGHT:
28,000
Product Source: Normal human plasma, fresh, non-frozen. Tested negative for HIV-1, HIV-2, HCV and HBc antibodies and HBsAg.
Physical form
Liquid in 10 mM NH4HCO3, pH 7.4. Non-sterile, no preservatives.
Storage and Stability
Maintain lyophilized material at < -20°C for up to six months. Maintain reconstituted Apo A-I at +4°C up to 4 weeks. Freezing solutions of apolipoproteins in the absence of denaturants may favor increased self-association or aggregation.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Apolipoprotein A-II content of human plasma high density lipoproteins measured by radioimmunoassay.
Schonfeld, G, et al.
Journal of Lipid Research, 18, 645-655 (1977)
Effect of pressure and ionic strength on the self-association of Apo-A-I from the human high density lipoprotein complex.
Formisano, S, et al.
The Journal of Biological Chemistry, 253, 354-359 (1978)
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