1.12588

Fluorocult^® Lauryl sulfate broth + MUG

according to ISO 11866, FDA-BAM, for Escherichia coli, for coliforms, suitable for microbiology

• Synonym(s): Coliform selective agar, Fluorocult^® Lauryl sulfate broth, Fluorocult^® Laurylsulfate-bouillon, LST-MUG Medium
• UNSPSC Code: 41106213

Properties

• Agency: FDA-BAM; GB 4789.36-2016; according to ISO 11866
• Quality Level: 200
• form: medium granules (dehydrated (DCM))
• packaging: pkg of 500 g
• manufacturer/tradename: FluoroCult^®
• storage condition: protect from light (Keep tightly closed and dry)
• technique(s): microbiological culture: suitable
• color: beige
• pH: 6.6-7.0 (25 °C, 36.5 g/L in H₂O, after autoclaving)
• solubility: 36.5 g/L
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: Enterobacter spp.; Escherichia coli; Klebsiella spp.; coliforms

Description

General description

Fluorocult^® Lauryl sulfate broth + MUG (acc. ISO 11866 and FDA-BAM) is used to examine Escherichia coli in food, milk, and milk products. The lauryl sulfate inhibits the growth of undesirable microbial flora. The presence of E. coli is indicated by fluorescence under a long-wavelength UV lamp. A positive indole reaction and gas formation due to the fermentation of lactose confirm the results.

Application

Fluorocult^® Lauryl sulfate broth + MUG is suitable for the examination of E. coli in food, milk, and milk products.

Features and Benefits

• Fluorocult^® fluorogenic culture media offer Rapid and Reliable culture testing
• Powerful alternatives to conventional microbiological methods for the rapid and sensitive detection of bacteria
• Offers superior granulated culture media
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands

Analysis Note

Appearance (clearness): clear
Appearance (color): yellowish to yellowish-brown
pH-value (25 °C): 6.6 - 7.0
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium(Escherichia coli NCTC 13216 (WDCM00202)): ≤ 100
Inoculum on reference medium (Citrobacter freundii ATCC 43864 (WDCM 00006)): ≤ 100
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 1E+04
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≥ 1E+04
Growth(Escherichia coli ATCC 8739 (WDCM 00012)): good to very good
Growth Escherichia coliATCC 25922 (WDCM 00013): good to very good
Growth (Escherichia coli NCTC 13216 (WDCM00202)): good to very good
Growth (Citrobacter freundii ATCC 43864 (WDCM 00006)): good to very good
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total to partial inhibition
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total to partial inhibition
Gas (Escherichia coli ATCC 8739 (WDCM 00012)): +
Gas (Escherichia coli ATCC 25922 (WDCM 00013)): +
Gas (Escherichia coli NCTC 13216 (WDCM 00202)): +
Gas (Citrobacter freundii ATCC 43864 (WDCM 00006)): +
Gas (Enterococcus faecalis ATCC 19433 (WDCM 00009)): -
Gas (Enterococcus faecalis ATCC 29212 (WDCM 00087)): -
Fluorescence (Escherichia coli ATCC 8739 (WDCM 00012)): +
Fluorescence (Escherichia coli ATCC 25922 (WDCM 00013)): +
Fluorescence (Escherichia coli NCTC 13216 (WDCM 00202)): +
Fluorescence (Citrobacter freundii ATCC 43864 (WDCM 00006)): -
Fluorescence (Enterococcus faecalis ATCC 19433 (WDCM 00009)): -
Fluorescence (Enterococcus faecalis ATCC 29212 (WDCM 00087)): -
Indole formation (Escherichia coli ATCC 8739 (WDCM 00012)): +
Indole formation (Escherichia coli ATCC 25922 (WDCM 00013)): +
Indole formation (Escherichia coli NCTC 13216 (WDCM 00202)): +
Indole formation (Citrobacter freundii ATCC 43864 (WDCM 00006)): -
Indole formation (Enterococcus faecalis ATCC 19433 (WDCM 00009)): -
Indole formation (Enterococcus faecalis ATCC 29212 (WDCM 00087)): -
Incubation: 24 ± 2 to 48 ± 2 hours at 30 ± 1 °C, aerobic

Legal Information

FLUOROCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2