15044AST

Astec^® CHIROBIOTIC^® V2 Chiral (5 μm) HPLC Columns

L × I.D. 25 cm × 21.2 mm, HPLC Column

• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: Astec^® CHIROBIOTIC^® V2 Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 21.2 mm
• material: stainless steel column
• Agency: suitable for USP L88
• description: HPLC column
• product line: Astec^®
• packaging: pkg of 1 ea
• manufacturer/tradename: Astec^®
• parameter: 0-45 °C temperature; 241 bar pressure (3500 psi)
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 25 cm × 21.2 mm
• matrix: High-purity silica gel particle platform; fully porous particle
• matrix active group: vancomycin phase
• particle size: 5 μm
• pore size: 200 Å
• operating pH range: 3.5-7.0
• separation technique: chiral

Description

General description

Neutral molecules, amides, acids, esters and amines show considerable enantioselectivity on these vancomycin-based CSPs. A wide variety of secondary and tertiary amines have been separated on the CHIROBIOTIC^® V in the polar ionic mode. The CHIROBIOTIC^® V has demonstrated many of the separation characteristics of protein-based stationary phases, but with exceptional stability and much higher sample capacity. Some chiral analytes have been resolved that have not been reported separated on any other chiral stationary phase. CHIROBIOTIC^® V and V2 differ in their bonding chemistry the pore size of the support particle, giving them different selectivity and preparative capacity.

• Bonded phase: Vancomycin
• Operating pH range: 3.5 - 7.0
• Particle diameter: 5, 10 or 16 μm
• Pore size: 100 Å (CHIROBIOTIC^® V) or 200 Å (CHIROBIOTIC^® V2)

CHIROBIOTIC FAQs
CHIROBIOTIC Reference Bibliography
Chiral Product Literature

Application

• Development and validation of an UFLC-MS/MS method for enantioselectivity determination of d,l-threo-methylphenidate, d,l-threo-ethylphenidate and d,l-threo-ritalinic acid in rat plasma and its application to pharmacokinetic study.: This study utilizes the Astec^® CHIROBIOTIC^® V2 Chiral HPLC Column to develop and validate a UFLC-MS/MS method for the enantioselective determination of various methylphenidate and ritalinic acid enantiomers in rat plasma. The column′s application in pharmacokinetic studies underscores its effectiveness in biomedical research, particularly in analyzing chiral drug molecules (Zhang et al., 2016).


• New high-performance liquid chromatography method for the determination of (R)-warfarin and (S)-warfarin using chiral separation on a glycopeptide-based stationary phase.: This research employs the Astec^® CHIROBIOTIC^® V2 Chiral HPLC Column for the chiral separation and determination of warfarin enantiomers. The study highlights the column′s utility in pharmaceutical analysis and its effectiveness in the study of chiral drug enantiomers (Malakova et al., 2009).

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Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany

CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC