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W4125

Sigma-Aldrich

Williams′ Medium E

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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About This Item

UNSPSC Code:
12352207

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.025 g/L
L-glutamine: 0.292 g/L
glucose: 2.0 g/L
phenol red: 0.0107 g/L
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

Related Categories

General description

Williams′ E medium is a rich medium developed by Williams′ and Gunn. It is recommended for supporting long-term adult liver epithelial cell culture.

Application

Williams′ E medium has been used in culturing mice/rat primary hepatocytes.

Quantity

Formulated to contain 10.8 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Etienne Guillocheau et al.
The Journal of nutritional biochemistry, 63, 19-26 (2018-10-14)
Trans-palmitoleic acid (trans-C16:1 n-7 or trans-Δ9-C16:1, TPA) is believed to improve several metabolic parameters according to epidemiological data. TPA may mainly come from direct intakes: however, data are inconsistent due to its very low amount in foods. Instead, TPA might
Christina P Keravnou et al.
Ultrasound in medicine & biology, 42(11), 2676-2686 (2016-08-25)
Localized drug delivery and uptake can benefit from the combined action of ultrasound and microbubbles at a specific site. Some of the possible mechanisms suggested are vessel poration and/or cell poration, but the exact acoustic parameters that trigger those phenomena
M Herranz-Itúrbide et al.
Redox biology, 40, 101841-101841 (2021-01-26)
Liver is a unique organ in displaying a reparative and regenerative response after acute/chronic damage or partial hepatectomy, when all the cell types must proliferate to re-establish the liver mass. The NADPH oxidase NOX4 mediates Transforming Growth Factor-beta (TGF-β) actions
Xue Yu et al.
Acta biochimica et biophysica Sinica, 41(12), 1027-1032 (2009-12-17)
Homocysteine is an intermediate in the sulfur amino acid metabolism. Recent studies suggested that there might be links between hyperhomocysteinemia and insulin resistance. In the present study, we investigated the effect of homocysteine on glucose metabolism. We demonstrated that the
D M Toivola et al.
Journal of cell science, 110 ( Pt 1), 23-33 (1997-01-01)
The importance of protein phosphatases in the maintenance of cytoskeletal structure is supported by the serious liver injury caused by microcystin-LR, a hepatotoxic inhibitor of type-1 and type-2A serine/threonine protein phosphatases. We used the microcystin-LR-induced cell injury as a model
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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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