T5393
Anti-Mouse IgG (whole molecule)−TRITC antibody produced in goat
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Goat Anti-Mouse IgG (whole molecule)−Tetramethylrhodamine isothiocyanate
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About This Item
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biological source
goat
conjugate
TRITC conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct immunofluorescence: 1:64
storage temp.
2-8°C
target post-translational modification
unmodified
General description
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Anti-Mouse IgG (whole molecule)-TRITC antibody is specific for mouse IgG and all subclasses, it cross reacts with mouse IgA and IgM. Antibody purification is by ion exchange chromatography after which it is conjugated to crystalline tetramethylrhodamine isothiocyanate (TRITC).
Anti-Mouse IgG (whole molecule)-TRITC antibody is specific for mouse IgG and all subclasses, it cross reacts with mouse IgA and IgM. Antibody purification is by ion exchange chromatography after which it is conjugated to crystalline tetramethylrhodamine isothiocyanate (TRITC).
Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other goat serum proteins. The antibody is conjugated to crystalline tetramethylrhodamine isothiocyanate (TRITC) and then further purified to remove free TRITC. Specificity for mouse IgG is determined by Ouchterlony Double Diffusion (ODD). The antibody preparation is specific for mouse IgG and all subclasses, it cross reacts with mouse IgA and IgM. Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion against anti-goat IgG and anti-goat whole serum results in single arcs of precipitation in the γ region.
Specificity
The antibody preparation is specific for mouse IgG and all subclasses, it cross reacts with mouse IgA and IgM.
Immunogen
IgG isolated from pooled normal mouse serum
Application
Anti-Mouse IgG (whole molecule)-TRITC antibody may be used for immunofluorescence at a minimum antibody dilution of 1:64 using mouse spleen cells. Antibody dilution range of 1:50 to 1:250 was used for immunofluorescence in various studies.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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