SRP6147
Ubiquitin Activating Enzyme E1 human
≥95% (SDS-PAGE)
Synonym(s):
AMCX1, POC20, UBA1, UBE1, Ubiquitin E1
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
biological source
human
Assay
≥95% (SDS-PAGE)
form
liquid
potency
>3 nM
mol wt
118
packaging
pkg of 50 μg
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... UBA1(7317)
General description
Research area: Cell Signaling
Ubiquitin activating enzyme 1 (UBA1) or UBE1, is an ubiquitin-activating enzyme. The gene encoding it is localized on human chromosome Xp11.23.
Ubiquitin activating enzyme 1 (UBA1) or UBE1, is an ubiquitin-activating enzyme. The gene encoding it is localized on human chromosome Xp11.23.
Application
Ubiquitin Activating Enzyme E1 human has been used in in vitro and in vivo ubiquitination assays.
Biochem/physiol Actions
Ubiquitin activating enzyme 1 (UBA1) has a role in cellular protein homeostasis. It is the first enzyme utilized in the pathway to conjugate ubiquitin onto target substrates through an ubiquitin-protein isopeptide bond. The C-terminal glycine of an ubiquitin (G76) is initially adenylated in an ATP dependent manner activating it so it can be transferred to the active site cysteine of UBE1 forming an ubiquitin-UBE1 thiolester. With the help of the other enzymes in the ubiquitin conjugation pathway (E2/E3 enzymes) the ubiquitin is transferred to a specific lysine on a target substrate. The protein has been associated with spinal muscular atrophy (SMA) and Huntington′s disease (HD).
Physical form
In 50 mM Tris (pH 8.0), 100 mM NaCl and 10% glycerol.
Preparation Note
Centrifuge the vial prior to opening.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
E2 enzymes: more than just middle men.
Stewart MD
Cell Research, 26(4), 423-440 (2016)
UBA1: At the Crossroads of Ubiquitin Homeostasis and Neurodegeneration.
Groen EJ and Gillingwater TH
Trends in Molecular Medicine, 21(10), 622-632 (2015)
Structure of a ubiquitin E1-E2 complex: insights to E1-E2 thioester transfer.
Olsen SK and Lima CD
Molecular Cell, 49(5), 884-896 (2013)
UBE1 expression in extranodal NK/T cell lymphoma, nasal type.
Z L Su et al.
Leukemia & lymphoma, 49(9), 1821-1822 (2008-07-29)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service