SRE0117
Salt Active Nuclease (SAN)
High Purity, recombinant, expressed in Pichia pastoris, Triton-free
Synonym(s):
endonuclease, salt active endonuclease, salt tolerant nuclease
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About This Item
Recommended Products
recombinant
expressed in Pichia pastoris
Quality Level
composition
Triton-free
packaging
pkg of 25 kU
shipped in
dry ice
storage temp.
−20°C
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General description
Application
- Useful for purification of tagged recombinant proteins
- Manufacturing of nucleic acid-free molecular enzymes and reagents
- Research of vaccines, viruses and antibodies
Features and Benefits
- Active at low temperatures
- Binds strongly to cation exchange media because of high isoelectric point, even at elevated pH and salt concentrations where few other proteins show binding
- Typically does not bind to anion exchange or IMAC media
- Detergent free No protease detected
Unit Definition
Reconstitution
Other Notes
- Temperature: 5 – 40°C, optimal: ~35°C
- Salt concentration (NaCl / KCl): 150 - 900 mM, optimal: 400 - 650 mM
- Mg2+: >1 mM is required for activity, optimal 5 - 50 mM
- pH: 7.3 – 10.0, optimal 8.2 - 9.2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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