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P4306

Sigma-Aldrich

Platelet-Derived Growth Factor-BB human

PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

PDGF-BB

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About This Item

MDL number:
MFCD00213023
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

human

Quality Level

200

recombinant

expressed in E. coli

Assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

1.0-3.0 ng/mL EC50

quality

endotoxin tested

mol wt

24.3 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg

UniProt accession no.

P01127

storage temp.

−20°C

Gene Information

human ... PDGFB(5155)

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General description

Platelet-Derived Growth Factor (PDGF), a cationic polypeptide, is an important serum mitogen predominantly found in cells of mesenchymal origin. PDGF is mapped to human chromosome 7p22.3 and is expressed in smooth muscle cells, endothelial cells and macrophages. PDGF exists as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.

Application

Platelet-Derived Growth Factor-BB human has been used in induction of DNA synthesis and proliferation in low-concentration serum.

Biochem/physiol Actions

Platelet-derived growth factor (PDGF) is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts. PDGF elicits multifunctional actions in a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells and chondrocytes. PDGF-BB isomer is highly expressed in tumor, mediating oncogenic pericyte recruitment and is is a key regulator of angiogenesis. PDGF-BB controls bone homeostasis, repair and regeneration. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins and modulates LDL receptor binding.

Other Notes

Dimer of the B chain of human PDGF.

Physical form

Lyophilized from a 0.2 μm filtered solution.

Analysis Note

The biological activity is measured by the dose-dependent stimulation of thymidine uptake in BALB/c 3T3 cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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NIH 3T3 cells stably transfected with the gene encoding phosphatidylcholine-hydrolyzing phospholipase C from Bacillus cereus acquire a transformed phenotype.
Johansen T, et al.
Molecular and Cellular Biology, 14.1, 646-654 (1994)
The platelet derived growth factor-B polymorphism is associated with risk of severe fever with thrombocytopenia syndrome in Chinese individuals
Zhang XA, et al.
Oncotarget, 7(22), 33340-33349 (2016)
The role of growth factors in wound healing
David L, et al.
The Surgical Clinics of North America, 77.3, 575-586 (1997)
Overexpression of platelet-derived growth factor-BB increases tumor pericyte content via stromal-derived factor-1a/CXCR4 axis
Song N, et al.
Cancer Research, 69.15, 6057-6064 (2009)
Growth factors in the treatment of early osteoarthritis
Civinini R, et al.
Clinical Cases in Mineral and Bone Metabolism : The Official Journal of the Italian Society of Osteoporosis, Mineral Metabolism, and Skeletal Diseases, 10.1, 26-26 (2013)
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Articles

Platelet Derived Growth Factors (PDGF)

Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.

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