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P2893

Sigma-Aldrich

JumpStart™ Taq ReadyMix™

Complete optimized reagent for hot-start PCR at 2X concentration

Synonym(s):

hot start PCR master mix, hot start master mix, hot start taq master mix, mutiplex PCR

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About This Item

MDL number:
MFCD00166026
UNSPSC Code:
41106300
NACRES:
NA.55

Quality Level

200

form

liquid

usage

sufficient for 100 reactions
 sufficient for 400 reactions

feature

Multiplex PCR
dNTPs included
hotstart

concentration

2.5 units/reaction (50 μL reaction volume)

technique(s)

PCR: suitable

color

colorless

suitability

suitable for (quantitative PCR)

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

General description

JumpStart™ Taq ReadyMix™ is a ready-to-use 2X master mix that contains JumpStart™ Taq DNA polymerase, 99% pure dNTPs, reaction buffer and JumpStart™ Taq antibody. The Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.

Application

JumpStart™ Taq ReadyMix™ has been used in quantitative polymerase chain reaction (PCR). It has also been used to identify fibroblast cell surface adenosine receptor (AR) types under hypoxia. It is also suitable:
  • For PCR amplifications that require reduced non-specific amplification
  • For multiplex PCR
  • For reduction of primer dimers

Features and Benefits

  • The master mix allows consistency from one reaction to the next
  • Reduced preparation time and reduced risk of contamination from multiple pipetting steps
  • Designed to minimize non-specific amplification and contamination
  • Increased specificity and target yield
  • Reduced primer dimers
  • Reduced set-up time as compared to manual or wax Hot Start methods
  • Allows for room temperature set-up
  • Ideal for high throughput, quantitative PCR applications

Packaging

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL
400RXN is packaged as 1 X 10 mL

Other Notes

JumpStart Taq ReadyMix is a prepared solution combining the performance benefits of hot start PCR with the convenience of a ReadyMix. The mix includes JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and buffer in a 2× optimized reaction concentrate. JumpStart Taq Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Unlike other hot-start methods (i.e. chemical inactivation), JumpStart Taq polymerase does not require a pre-incubation step prior to cycling because polymerase activity is fully restored during the first denaturation cycle of the PCR reaction. The hot start mechanism allows for room temperature set up, making it ideal for high throughput applications. To prepare a 50 μL reaction, simply add 25 μL of ReadyMix to template, primers and water for a final reaction volume of 50 μL.
2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
To prepare a 50 μL reaction, simply add 25 μL of ReadyMix™ to template, primers and water for a final reaction volume of 50 μL.
This product has been validated in quantitative PCR, but may require supplementation with magnesium chloride solution, 25 mM, Catalog Number M8787, a suitable fluorescent probe, and, if desired, an internal reference dye, Catalog Number R4526. View more detailed information on JumpStart Taq ReadyMix products at www.sigma-aldrich.com/hotstart.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Koen Kole et al.
GigaScience, 6(10), 1-6 (2017-10-13)
Experience-dependent plasticity (EDP) is essential for anatomical and functional maturation of sensory circuits during development. Although the principal synaptic and circuit mechanisms of EDP are increasingly well studied experimentally and computationally, its molecular mechanisms remain largely elusive. EDP can be
Effects of A2BR on the biological behavior of mouse renal fibroblasts during hypoxia
Tang Jin, et al.
Molecular Medicine Reports, 11(6), 4397-4402 (2015)
Valerie F Boltz et al.
Retrovirology, 13(1), 87-87 (2016-12-22)
Although next generation sequencing (NGS) offers the potential for studying virus populations in unprecedented depth, PCR error, amplification bias and recombination during library construction have limited its use to population sequencing and measurements of unlinked allele frequencies. Here we report
Diego Ottaviani et al.
Genome research, 18(11), 1778-1786 (2008-10-14)
The folding of chromatin into topologically constrained loop domains is essential for genomic function. We have identified genomic anchors that define the organization of chromatin loop domains across the human major histocompatibility complex (MHC). This locus contains critical genes for
S Kwok et al.
Nature, 339(6221), 237-238 (1989-05-18)
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.
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Articles

Multiplex qPCR with JumpStart™ Taq ReadyMix™ for Quantitative PCR

Method outlines use of a hot start Taq for multiplex qPCR and provides guidance on how to optimize dNTPs, primer, probes and MgCL2 concentrations. By optimizing these parameters, the user can improve assay sensitivity and linear range of detection.

Introduction and Historical Timelines

Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.

Quantitative PCR Basics

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

Hot Start PCR

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

Protocols

Recombinant eSpCas9 Protein for RNP-Based Genome Editing

The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system was discovered in bacteria, where it functions as an adaptive immune system against invading viral and plasmid DNA.

End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Antibody-Enzyme Mediated Hot Start PCR Protocol

When using hot start Taq DNA polymerase, the enzyme remains inactive until heated. Hot Start DNA polymerase control is achieved by chemical or antibody modification of the enzyme.

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