P1851

PIPES

BioPerformance Certified, suitable for cell culture

• Synonym(s): 1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)
• Empirical Formula (Hill Notation): C₈H₁₈N₂O₆S₂
• CAS Number: 5625-37-6
• Molecular Weight: 302.37
• Beilstein: 817713
• EC Number: 227-057-6
• MDL number: MFCD00006159
• UNSPSC Code: 12161700
• PubChem Substance ID: 24898249
• NACRES: NA.25

Properties

• grade: BioPerformance Certified; for molecular biology
• form: crystalline powder
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin and Total Aerobic Microbial Count, tested
• useful pH range: 6.1-7.5
• pKa (25 °C): 6.8
• mp: >300 °C (lit.)
• solubility: 1 M NaOH: 20 + 80 mL g, clear, colorless; soluble
• application(s): diagnostic assay manufacturing
• foreign activity: DNAse, Exonuclease; NICKase, Endonuclease; RNAse; Protease, none detected
• SMILES string: OS(=O)(=O)CCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)
• InChI key: IHPYMWDTONKSCO-UHFFFAOYSA-N

Description

Application

• Sensitive Detection of Immunoglobulin G Stability Using in Real-Time Isothermal Differential Scanning Fluorimetry: Determinants of Protein Stability for Antibody-Based Therapeutics.: The use of PIPES buffer in this study was integral to maintaining the stability of immunoglobulin G during differential scanning fluorimetry. This method is crucial for evaluating the stability of antibody-based therapeutics (Moggridge et al., 2017).

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves