N1038

Anti-Nup98 antibody, Rat monoclonal

clone 2H10, purified from hybridoma cell culture

• Synonym(s): Anti-Nucleoporin, 98-kD
• MDL number: MFCD09265425
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2H10, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~98 kDa
• species reactivity: rat, human
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable; indirect ELISA: suitable; western blot: 0.5-1 μg/mL using HeLa nuclear extract
• isotype: IgG2cκ
• UniProt accession no.: P52948
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NUP98(4928) ; rat ... Nup98(81738)

Description

General description

Monoclonal Anti-Nup98 (rat IgG2c- isotype) is derived from the hybridoma 2H10 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from WKY/NCrj rat immunized with a recombinant fragment of human Nup98. Nup96 and Nup98 are the nucleoporins that are formed by the cleavage of 186 kDa precursor protein.

Nucleoporin-98kDa (Nup98) is part of the nuclear pore complex. It possesses two domains and the gene encoding it is localized on human chromosome 11.

Application

Anti-Nup98 antibody, Rat monoclonal may be used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry

Biochem/physiol Actions

Nucleoporin-98kDa (Nup98) is involved in the translocation of RNA and proteins across the nucleus. It is also a regulator of chromosomal segregation and gene expression. The protein also binds to other nucleoporins and is associated with acute myelogenous leukemia.

Nup98 functions as a docking protein for cytosol mediated docking of import substrates. Securin protein damage caused by the anaphase-promoting complex (APC) during mitosis, is regulated by the nucleocytoplasmic transport factors Rae1 and Nup98. In mice, collective haploinsufficiency of Rae1 and Nup98 gene causes premature separation of sister chromatids and severe aneuploidy. In addition, it also leads to untimely degradation of securin. Chromosome rearrangement between the HOXA9 (homeobox A9) gene and the nucleoporin gene Nup98 has been observed in some human myeloid leukemia patients.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves