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M7403

Sigma-Aldrich

MCDB 153 Medium

With trace elements, L-glutamine and 28mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Molecular, Cellular, and Development Biology 153 medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)

shipped in

ambient

storage temp.

2-8°C

Related Categories

General description

MCDB 153 media are modifications of Ham′s nutrient mixture F-12, designed for the growth of human keratinocytes, clonal growth of chicken embryo fibroblasts, and Chinese hamster ovary (CHO) cells using low levels of fetal bovine serum protein (FBSP), extensive trace elements or no serum protein.

Application

MCDB 153 Medium has been used as a component of melanoma isolation media (MIM) to culture human non-metastatic and metastatic melanoma cells.

Quantity

Formulated to contain 17.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.18 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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