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Key Documents

GW20091F

Sigma-Aldrich

Anti-Luciferase antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Luciferin 4-Monooxygenase

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

chicken

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

firefly

manufacturer/tradename

Genway 15-288-20091F

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Photinus pyralis ... Loc116160065(116160065)

Immunogen

Immunogen Sequence: GI # 126501, sequence 1-550
Recombinant luciferin 4-monooxygenase (Luciferase)

Application

Anti-Luciferase antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Biochem/physiol Actions

Luciferase is a member of the class of oxidative enzymes essential for bioluminescence. The term is originated from Lucifer; one example is firefly luciferase from the firefly Photinus pyralis. Its unique properties have spawned a number of novel applications of this protein. Luciferase has a number of applications such as a reporter gene, as a model for the study of protein import into peroxisomes and as a component of a heterologous gene expression system. Its structure reveals a new protein fold, which may act as a representative of a growing family of homologous enzymes.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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T O Baldwin
Structure (London, England : 1993), 4(3), 223-228 (1996-03-15)
The structure of firefly luciferase reveals a new protein fold which may be representative of a growing family of homologous enzymes.
S J Gould et al.
Analytical biochemistry, 175(1), 5-13 (1988-11-15)
The unique properties of firefly luciferase and the cloning of the gene for this enzyme have spawned a number of novel applications of this protein. We summarize a few of these applications including its use as a reporter gene, as

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