GERPN2232
ECL™ Prime Western Blotting System
Cytiva RPN2232
Synonym(s):
Amersham™ ECL™ Prime, Chemiluminescent Western blotting kit, chemiluminescent detection reagent
About This Item
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packaging
pkg of 1 kit
manufacturer/tradename
Cytiva RPN2232
color
Detection reagent 1: Colorless Detection reagent 2: Colorless
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General description
Amersham™ ECL™ Prime consolidates and builds on the benefits of Amersham™ ECL™ Plus and Amersham™ ECL™ Advance to deliver a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents.
Application
Features and Benefits
- High signal intensity and sensitivity allows the use of highly diluted primary and secondary antibodies with no reduction in sensitivity.
- Stable signal allows multiple exposures and makes the reagent suitable for large experimental series, allowing convenient handling time between the end of the experiment and detection.
- Optimized for imaging with ImageQuant LAS series (CCD-based imaging) and compatible with Amersham™ Hyperfilm.
- Compatible with Rainbow™ Molecular Weight Markers and Amersham™ ECL™ DualVue Western Blotting Markers.
- Optimized for use with Amersham™ Hybond-P (PVDF) membranes and compatible with Amersham™ Hybond-ECL™ (nitrocellulose) membranes.
- Recommended membrane Hybond-P or Hybon ECL™
Components
- RPN2232 Solution A: Luminol solution, 50 ml Solution B: Peroxide solution, 50 ml sufficient for 1000 cm2 membrane
Storage and Stability
Analysis Note
Other Notes
Detection reagent 1: colorless
Detection reagent 2: colorless
Sensitivity: ~1 pg
Legal Information
This product or portions thereof is manufactured and sold under license from Cyanagen Srl and is subject of US patent 7,855,287, US Patent 7,803,573, and Italian application number TO2010A000580, together with other equivalent granted patents and patent applications in other countries.
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Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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