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GE17-0780-01

Protein A-Sepharose™ CL-4B

Cytiva 17-0780-01, pack of 1.5 g

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

native protein A

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 1.5 g

manufacturer/tradename

Cytiva 17-0780-01

storage condition

(20% Ehtanol)

matrix

4% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

2-10(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)

working range

3-9

capacity

~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

storage temp.

2-8°C

Related Categories

General description

Protein A Sepharose™ CL-4B is a classic, well documented medium for immunoglobin purification and fractionation, and is suitable for immunoprecipitation procedures.

Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.

Features and Benefits

  • Well documented for immunoglobulin purification and fractionation
  • Strong binding to Fc region of IgG
  • High capacity - 20 mg human IgG/mL.
  • Suited for immunoprecipitation procedures

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Daniel L Galvan et al.
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Fetuin-A (Fet-A) is a liver-secreted phosphorylated protein, known to impair insulin signaling, which has been shown to be associated with obesity, insulin resistance, and incident diabetes. Fet-A interacts with the insulin-stimulated insulin receptor (IR) and inhibits IR tyrosine kinase activity
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Ana Isabel de Los Santos-Velázquez et al.
Current biology : CB, 27(21), 3248-3263 (2017-10-24)
The nucleolus plays a pivotal role in multiple key cellular processes. An illustrative example is the regulation of mitotic exit in Saccharomyces cerevisiae through the nucleolar sequestration of the Cdc14 phosphatase. The peculiar structure of the nucleolus, however, has also
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