G4134
Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)
Type IX, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)
Synonym(s):
G-6-P-DH, Zwischenferment
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About This Item
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type
Type IX
Quality Level
form
lyophilized powder
specific activity
200-400 units/mg protein (modified Warburg-Christian)
mol wt
128 kDa
β-NADP and β-NADPH content
≤10 mmol/mol
application(s)
agriculture
shipped in
dry ice
storage temp.
−20°C
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General description
Research area: Cell Signaling
Glucose-6-phosphate dehydrogenase (G6PD) is a key metabolic enzyme of the pentose phosphate pathway. In S. cerevisiae, it is encoded by the ZWF1 gene. G6PD exists as a tetramer in its active form.
Glucose-6-phosphate dehydrogenase (G6PD) is a key metabolic enzyme of the pentose phosphate pathway. In S. cerevisiae, it is encoded by the ZWF1 gene. G6PD exists as a tetramer in its active form.
Application
Glucose-6-phosphate dehydrogenase is used:
- To test ketose reductase activity in developing maize endosperm.
- For recycling microassay of β-NADP and β-NADPH.
- To measure the intracellular levels of NADPH and total NADP.
- To measure the nicotinamide adenine dinucleotide (NAD) kinase kinetic assay activity.
Biochem/physiol Actions
Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.
Glucose-6-phosphate dehydrogenase catalyzes the rate-limiting step in the pentose phosphate pathway. Its function involves the conversion of glucose-6-phosphate to 6-phosphogluconolacetone while generating NADPH, which is essential for the regeneration of glutathione The glutathione system utilizes nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) to effectively eliminate excess hydrogen peroxide. Glucose-6-phosphate dehydrogenase (G6PD) plays an important role in regulating cell growth and survival. Their levels are higher in cells undergoing normal and neoplastic growth. Increased glucose-6-phosphate dehydrogenase activity plays a pivotal role in preventing reactive oxygen species mediated cell death. Glucose-6-phosphate dehydrogenase is over expressed in several cancers whereas its activity is reduced in hyperglycemia. A deficiency in glucose-6-phosphate dehydrogenase causes hemolysis.
Unit Definition
One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.
Physical form
Lyophilized powder essentially sulfate-free, containing approx. 20% sodium citrate
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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D Oh et al.
Molecular and cellular biology, 10(4), 1415-1422 (1990-04-01)
The Saccharomyces cerevisiae GAL5 (PGM2) gene was isolated and shown to encode the major isozyme of phosphoglucomutase. Northern (RNA) blot hybridization revealed that the GAL5 transcript level increased three- to fourfold in response to galactose and was severely repressed in
An improved cycling assay for nicotinamide adenine dinucleotide.
C Bernofsky et al.
Analytical biochemistry, 53(2), 452-458 (1973-06-01)
A simple ultramicro method for determination of pyridine nucleotides in tissues.
J S Nisselbaum et al.
Analytical biochemistry, 27(2), 212-217 (1969-02-01)
Pranavi Koppula et al.
Nature communications, 13(1), 2206-2206 (2022-04-24)
Targeting ferroptosis, a unique cell death modality triggered by unrestricted lipid peroxidation, in cancer therapy is hindered by our incomplete understanding of ferroptosis mechanisms under specific cancer genetic contexts. KEAP1 (kelch-like ECH associated protein 1) is frequently mutated or inactivated
D C Doehlert
Plant physiology, 84(3), 830-834 (1987-07-01)
Ketose reductase (NAD-dependent polyol dehydrogenase EC 1.1.1.14) activity, which catalyzes the NADH-dependent reduction of fructose to sorbitol (d-glucitol), was detected in developing maize (Zea mays L.) endosperm, purified 104-fold from this tissue, and partially characterized. Product analysis by high performance
Protocols
Enzymatic Assay of Hexokinase
This procedure may be used for all Phosphoglucomutase products except for Phosphoglucomutase, Catalog Number P4109.
Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)
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