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G0154

Sigma-Aldrich

Guillard′s (F/2) Marine Water Enrichment Solution

50 ×, liquid, suitable for plant cell culture

Synonym(s):

seawater medium

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About This Item

UNSPSC Code:
10171502
NACRES:
NA.72

sterility

sterile-filtered

Quality Level

400

form

liquid

concentration

50 ×

technique(s)

cell culture | plant: suitable

application(s)

agriculture

shipped in

dry ice

storage temp.

−20°C

General description

Guillard′s (F/2) Marine Water Enrichment Solution 50 X is a concentrate of major nutrients, trace metals, and vitamins that can be diluted in filtered seawater to support the reconstitution of Guillard′s (F/2) Marine Enrichment medium. Guillard′s (F/2) Marine Enrichment medium is a widely used enriched seawater medium for growing coastal marine algae (diatoms).

Application

Guillard′s (F/2) Marine Water Enrichment Solution has been used:
  • for culturing Chromera velia CCMP2878 to study coral response to Chromera infection
  • to enrich Baltic Sea water to culture unialgal stock of Symbiodinium strains
  • as a supplement in water culture medium to culture Chaetoceros affinis (CA) inoculum

Guillard′s (F/2) Marine Water Enrichment Solution 50 X, is a concentrate of major nutrients, trace metals and vitamins that can be diluted in filtered seawater to support reconstitution of Guillard′s (F/2) Marine Enrichment medium. Guillard′s (F/2) Marine Enrichment medium is a widely used enriched seawater medium for growing costal marine algae (diatoms).

Formula variant

Without silicate. With the major nutrients, trace metals and vitamins as described by Guillard (1975).

Reconstitution

Use at 20ml/L of seawater.

Phycology Media composition and preparation from powder

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Girish Beedessee et al.
Scientific reports, 9(1), 1204-1204 (2019-02-06)
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Protocols

Phycology Media in Plant Tissue Culture

Phycology media can be prepared from concentrated solutions or from powdered salt mixtures. The concentrated solutions are complete, including vitamins, and should be stored frozen.

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