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DUO92105

Sigma-Aldrich

Duolink® In Situ Red Starter Kit Goat/Rabbit

Synonym(s):

in situ Proximity Ligation Assay Kit, Protein Protein Interaction Kit

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

product line

Duolink®

technique(s)

proximity ligation assay: suitable

fluorescence

λex 594 nm; λem 624 nm (red) (Texas Red®; Zeiss Filter set 31)

suitability

suitable for fluorescence

Application

Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.

To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes.This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Goat PLUS and Anti-Rabbit MINUS), red detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
The Duolink® In Situ Red Starter Kit Goat/Rabbit requires one primary antibody from goat and one primary antibody from rabbit. Red fluorescence detection reagents are often used with Texas Red® filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

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Features and Benefits

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Legal Information

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
Texas Red is a registered trademark of Life Technologies

Kit Components Also Available Separately

Product No.
Description
SDS
  • DUO92005Duolink® In Situ PLA® Probe Anti-Rabbit MINUSSDS
  • DUO92003Duolink® In Situ PLA® Probe Anti-Goat PLUSSDS
  • DUO92008Duolink® In Situ Detection Reagents RedSDS
  • DUO82040Duolink® In Situ Mounting Medium with DAPISDS
  • DUO82049Duolink® In Situ Wash Buffers, FluorescenceSDS

Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Danger

Hazard Statements

H290,H334,H412

Hazard Classifications

Aquatic Chronic 3 - Met. Corr. 1 - Resp. Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Shaida Ouladan et al.
International journal of oncology, 46(6), 2595-2605 (2015-04-23)
Solitary fibrous tumors (SFTs) are rare mesenchymal neoplasms, displaying variable morphological and clinicopathological features. Supportive immunohistochemical markers such as CD34, CD99, BCL2 and LSD1 are commonly applied in the differential diagnosis of SFTs, although none is sufficiently sensitive or specific
Linbo Wang et al.
Journal of cancer research and clinical oncology, 140(12), 1997-2008 (2014-07-10)
Our previous work identified leucine zipper transcription factor-like 1 (LZTFL1) as a novel tumor suppressor gene, with its expression correlated with survival outcome in gastric cancer (GC) patients. This study focuses on the role of LZTFL1 in GC aggression and
Gunisha Sagar et al.
Gut, 65(7), 1165-1174 (2015-06-11)
New-onset diabetes and concomitant weight loss occurring several months before the clinical presentation of pancreatic cancer (PC) appear to be paraneoplastic phenomena caused by tumour-secreted products. Our recent findings have shown exosomal adrenomedullin (AM) is important in development of diabetes
Dipti Pravin Lambade et al.
Journal of clinical and diagnostic research : JCDR, 9(2), ZC01-ZC05 (2015-04-11)
The purpose of this vitro study was to comparatively evaluate the adhesive bonding of dual cured resin luting agents with lithium disilicate ceramic material. Porcelain laminate veneers were prepared with lithium disilicate ceramic material i.e. IPS Empress II( E-Max Press).
Seo-Young An et al.
Journal of prosthodontics : official journal of the American College of Prosthodontists, 24(8), 642-646 (2015-04-14)
This study examined the radiopacity of contemporary luting cements using direct digital radiography under a range of exposure conditions. Disc specimens (N = 80, n = 10 per group, ø5 mm × 1 mm) were prepared from 8 resin-based luting
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Protocols

Duolink® In Situ Short Instructions - Fluorescence

This page details the Duolink® In Situ Short Protocol for fluorescence detection

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