Neuro 2a Cell Line from mouse

89121404

• Synonym(s): N2a Cells, Nb2a Cells, Neuro2a Cells
• UNSPSC Code: 41106514

Properties

• biological source: mouse nerve
• description: Mouse Albino neuroblastoma
• growth mode: Adherent
• karyotype: 2n = 40
• morphology: Neuronal/amoeboid-like
• products: Microtubular protein
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse Albino neuroblastoma

Cell Line Description

Derived from a spontaneous tumour in an albino strain A mouse. Cells produce microtubular protein which is believed to play a role in the contractile system giving axoplasmic flow in nerve cells.

Application

Neuro 2a cell line has been used as a model to study the potential toxicological effects of copper oxide nanoparticles accumulation in the brain. It has also been used to determine the sensitivity of the cell line to saxitoxin.

Tumourigenicity studies, microtubular protein synthesis, vinblastine precipitation, GTP binding kinetics

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 4x10,000 cells cm²; 5% CO₂; 37°C. After resuscitation cells can take up to 6 days before a monolayer forms. These cells only adhere to the culture flask very lightly, care should be taken when subculturing. There is no need to wash the cells with PBS or trypsin, merely pour off half of the spent medium carefully, give the flask a gentle knock and resuspend the cells in fresh medium using a pipette to gently disperse any clumps of cells to finally reseed at 1:2. On arrival, growing cultures should be spun out and re-seeded in fresh media.

Other Notes

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