AFRC MAC 57
86030310, Reactivity: Anti-Lipopolysaccharide
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About This Item
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biological source
Rat x Rat Hybridoma
ImmunoDon
LOU/Iap rat spleen
Myeloma
Y3Ag1.2.3
Reactivity
Anti-Lipopolysaccharide
technique(s)
cell culture | mammalian: suitable
isotype
IgM
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat Y3Ag1.2.3 x Rat LOU/Iap spleen
Cell Line Description
The antibody is directed against lipopolysaccharide components of the bacterial cell wall and also with lipopolysaccharide from free-living cultures of Rhizobium strains 3841 and B155.
Immunogen
Sonicated bacteroid material from Rhizobium leguminosarum strain 3841 and strain B155
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
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