Recommended Products
biological source
mouse unknown/unspecified
growth mode
Suspension
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Mouse BALB/c (H-2d) T cells x Mouse AKR Thyoma, BW5147
Cell Line Description
Do-11-11 was produced by fusion of the antigen-specific I region-restricted (H-2d) BALB/c T-cell blasts with the AKR thyoma BW5147. This murine helper T cell hybridoma is specific for chicken ovalbumin (cOva) restricted to I-Ad and cross reacts with cOva I-antibody. The hybrid can be stimulated to secrete Interleukin 2 (IL-2) or to act as an antigen-specific helper T-cell. Expression of IL-2 can be lost by long-term culture of growth at high densities.
Culture Medium
DMEM or RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37 °C. Cells should not be grown at high density; avoid long-term culture as this might lead to change of cell receptors.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service