C8546

Cellulase from Trichoderma reesei ATCC 26921

lyophilized powder, ≥1 unit/mg solid

• Synonym(s): 1,4-(1,3:1,4)-β-D-Glucan 4-glucano-hydrolase
• CAS Number: 9012-54-8
• Enzyme Commission number: 3.2.1.4 (BRENDA, IUBMB)
• EC Number: 232-734-4
• MDL number: MFCD00081510
• UNSPSC Code: 12352204

Properties

• form: lyophilized powder
• Quality Level: 200
• specific activity: ≥1 unit/mg solid
• greener alternative product characteristics: Waste Prevention; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• solubility: deionized water: soluble 5.0 mg/mL (Sterile; In the presence of 0.15% polyhexamethylene biguanide (PHMB).)
• greener alternative category: Enabling,
• storage temp.: 2-8°C

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.

Application

Cellulase from Sigma has been used to degrade cello-oligosaccharides into glucose; to study the biodegradability of bioabsorbable bacterial cellulose (BBC) material.

Cellulase from Trichoderma reesei has been used in a study to assess the differential regulation of the cellulase transcription factors XYR1, ACE2, and ACE. Cellulase from Trichoderma reesei has also been used in a study to investigate the liquefaction of hydrothermally pretreated wheat straw at high-solids content.

Biochem/physiol Actions

Cellulases are enzymes that hydrolyze cellulose to glucose. Cellulase is used to study the development of occupational asthma in the detergent, pharmaceutical, baking, and enzyme production industries. Cellulase is added to detergents to improve cleansing properties. It is also applied in the biodegradation of paper products such as foolscap paper, filter paper, newspaper and office paper as well as microcrystalline cellulose.

Unit Definition

One unit will liberate 1.0 μmole of glucose from cellulose in one hr at pH 5.0 at 37 °C (2 hr incubation time).

Preparation Note

Dissolves in sterile deionized (DI) water in the presence of 0.15% polyhexamethylene biguanide (PHMB) at 5 mg/mL concentration.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P304 + P340 - P342 + P311
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves