C6295
Cell Freezing Medium-DMSO Serum free 1x
sterile-filtered, suitable for cell culture
Synonym(s):
cryopreservation medium
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Recommended Products
sterility
sterile-filtered
Quality Level
form
liquid
technique(s)
cell culture | mammalian: suitable
cryopreservation: suitable
impurities
endotoxin, tested
shipped in
dry ice
storage temp.
−20°C
General description
Cell freezing medium is a combination of cryoprotectant DMSO with fetal bovine serum. However serum free medium replaces serum with methylcellulose and poloxamer 188 and other additives like ascorbic acid, α-tocopherol and proline.
Application
Cell Freezing Medium-DMSO Serum free 1x has been used in the freezing of human neural progenitor cells (hNPCs), olfactory ensheathing cells (OECs) and retinal pigment epithelial (RPE) cells.
Other Notes
Complete ready-to-use medium containing Minimum Essential Medium, dimethyl sulfoxide (8.7%) and methyl cellulose. Prepared according to the formulation of Waymouth.
related product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Acute and delayed transplantation of olfactory ensheathing cells promote partial recovery after complete transection of the spinal cord
Lopez-Vales R, et al.
Neurobiology of Disease, 21(1), 57-68 (2006)
Simple freezing procedure for storage in serum-free media of cultured and tumor cells of mouse.
Waymouth, C. and Varnum, D.
TCA Manual, 2(1), 311-313 (1976)
Cryopreservation of cells using defined serum-free cryoprotective agents
Volbers JC, et al.
Current Directions in Biomedical Engineering, 2(1), 315-318 (2016)
A novel serum-free method for culturing human prenatal retinal pigment epithelial cells
Gamm DM, et al.
Investigative Ophthalmology & Visual Science, 49(2), 788-799 (2008)
Glial cell line-derived neurotrophic factor-secreting human neural progenitors show long-term survival, maturation into astrocytes, and no tumor formation following transplantation into the spinal cord of immunocompromised rats.
Gowing G, et al.
Neuroreport, 25(6), 367-367 (2014)
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