C4018

Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)

lyophilized powder, ≥100 units/mg protein

• Synonym(s): 3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase
• CAS Number: 37289-07-9
• Enzyme Commission number: 3.5.1.24 (BRENDA, IUBMB)
• MDL number: MFCD00130789
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: lyophilized powder
• specific activity: ≥100 units/mg protein
• composition: Protein, ≥50% biuret
• shipped in: wet ice
• storage temp.: −20°C

Description

Application

The enzyme from Sigma has been used in the analysis of bile samples in various studies.

Biochem/physiol Actions

The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg²⁺, Cu²⁺, and Cd²⁺) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.

Unit Definition

One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.

Physical form

Partially purified lyophilized powder containing buffer salts and stabilizer

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves