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B9638

Sigma-Aldrich

Basal Medium Eagle

With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

BME

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: 0.011 g/L
NaHCO3: no
L-glutamine: 0.292 g/L
Earle’s salts (5% CO2): yes

shipped in

ambient

storage temp.

2-8°C

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Application

BME, when properly supplemented, has demonstrated wide applicability, for supporting monolayer growth of a wide variety of normal and transformed cell lines.

Quantity

Formulated to contain 9.2 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Other Notes

Eagle's Basal Medium or BME, developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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