A7481

Anti-Atg1/ULK1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-UNC51, Anti-Unc-51 like kinase 1 (C. elegans), Anti-Unc51.1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~150 kDa
• species reactivity: rat, human (predicted), mouse
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1 mg/mL
• technique(s): western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells expressing mouse ULK1; western blot: 3-6 μg/mL using whole extracts of rat PC12
• UniProt accession no.: O75385
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ULK1(8408) ; mouse ... Ulk1(22241) ; rat ... Ulk1(360827)

Description

General description

Atg1 is an autophagy protein that functions as a serine/threonine kinase in yeast to regulate autophagasome formation. Atg1 is ULK1 in mammals.

Unc-51 like autophagy activating kinase 1 (ULK1) gene is located on human chromosome 12q24.33.

Specificity

Anti- Atg1/ULK1 recognizes rat and mouse Atg1/ULK1.

Immunogen

Synthetic peptide corresponding to amino acids of mouse Atg1/ULK1 conjugated to KLH via a cysteine residue. The corresponding sequence is identical in rat and differs by 3 amino acids in human.

Application

Anti-Atg1/ULK1 antibody produced in rabbit has been used in western blotting and immunohistochemical staining.

Biochem/physiol Actions

Unc-51 like autophagy activating kinase 1 (ULK1) regulates autophagy initiation and innate immunity of interferons (IFNs). ULK1 is critical for neuronal development. It is essential for neurite elongation and differentiation of cerebellar granule neurons. Mutations in this gene is linked to latent mycobacterium tuberculosis infection (LTBI).

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves