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85L2

Sigma-Aldrich

Leukocyte Alkaline Phosphatase Kit

based on naphthol AS-MX phosphate and fast blue RR salt (with citrate)

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About This Item

UNSPSC Code:
12352106
NACRES:
NA.47

Quality Level

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

application(s)

hematology
histology

shipped in

wet ice

storage temp.

2-8°C

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Application

Leukocyte Alkaline Phosphatase (LAP) kits are intended for the qualitative demonstration of alkaline phosphatase activity in white blood cells.
Peripheral blood or bone marrow preparations are fixed to a microscope slide. The film is then incubated in a mixture of naphthol AS-MX phosphate alkaline solution with fast blue RR salt. The resulting blue, insoluble, granular dye deposit indicates sites of alkaline phosphatase activity.

Kit Components Also Available Separately

Product No.
Description
SDS

  • MHS1Mayer′s Hematoxylin Solution 100 mLSDS

  • 855Naphthol AS-MX Phosphate Alkaline Solution, 0.25% 20 mLSDS

  • 854CCitrate Concentrated Solution 20 mLSDS

  • FBS25Fast Blue RR Salt, Preweighed Capsules 10 capsulesSDS

Pictograms

CorrosionExclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8A - Combustible corrosive hazardous materials


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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G Bouet et al.
European cells & materials, 29, 250-266 (2015-05-16)
An engineered three dimensional (3D) in vitro cell culture system was designed with the goal of inducing and controlling in vitro osteogenesis in a reproducible manner under conditions more similar to the in vivo bone microenvironment than traditional two-dimensional (2D)
Luca Azzolin et al.
Cell, 151(7), 1443-1456 (2012-12-19)
Wnt growth factors are fundamental regulators of cell fate, but how the Wnt signal is translated into biological responses is incompletely understood. Here, we report that TAZ, a biologically potent transcriptional coactivator, serves as a downstream element of the Wnt/β-catenin
Guenaelle Bouet et al.
PloS one, 10(2), e0117402-e0117402 (2015-02-25)
Bone sialoprotein (BSP) belongs to the "small integrin-binding ligand N-linked glycoprotein" (SIBLING) family, whose members interact with bone cells and bone mineral. BSP is strongly expressed in bone and we previously showed that BSP knockout (BSP-/-) mice have a higher
Clarissa R Taufer et al.
The International journal of developmental biology, 64(7-8-9), 433-443 (2020-10-17)
The neural crest (NC) is a transitory embryonic structure of vertebrates that gives rise to an astonishing variety of derivatives, encompassing both neural and mesenchymal cell types. Neural crest cells (NCCs) are an excellent model to study how environmental factors
Haibo Zhao et al.
Developmental cell, 14(6), 914-925 (2008-06-10)
Maintenance of bone mass and integrity requires a tight balance between resorption by osteoclasts and formation by osteoblasts. Exocytosis of functional proteins is a prerequisite for the activity of both cells. In the present study, we show that synaptotagmin VII

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