11579681001
Roche
RNase, DNase-free, High Concentration
from bovine pancreas
Synonym(s):
Rnase
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About This Item
UNSPSC Code:
41105600
biological source
bovine pancreas
Quality Level
form
solution
specific activity
≥30 U/mg
packaging
pkg of 1 mg (10 mg/ml)
manufacturer/tradename
Roche
concentration
10 mg/mL
General description
A heterogeneous mixture of ribonucleases specially prepared to be free of deoxyribonuclease activity according to the current Quality Control procedures.
Application
High concentration, DNase-free RNase has been used for:
- Genomic DNA isolation
- Isolation of plasmid DNA
- Degradation of RNA for flow cytometry
Quality
Absence of DNase activity tested according to the current Quality Control procedures.
Unit Definition
One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under assay conditions: A0 to A1 corresponds to the total conversion, A1 being the final absorbance.
Preparation Note
Working concentration: 50 μg/ml is the recommended working concentration of RNase, DNase-free, for the isolation of genomic DNA.
Working solution: Recommended in dilution buffer: 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (v/v), pH 7.0
Working solution: Recommended in dilution buffer: 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (v/v), pH 7.0
Other Notes
For life science research only. Not for use in diagnostic procedures.
Unlike most RNase preparations, no boiling before use is necessary to remove DNase activity.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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