PRCYTA-40K
MILLIPLEX® Non-Human Primate Cytokine/Chemokine/Growth Factor Panel A
Synonym(s):
Luminex® non-human primate cytokine immunoassay, Millipore non-human primate cytokine panel, Monkey cytokine multiplex kit
About This Item
Recommended Products
species reactivity
nonhuman primates
technique(s)
multiplexing: suitable
assay range
inter-assay cv: <20%
intra-assay cv: <10%
inter-assay cv: <30%
(TFN-α)
detection method
fluorometric (Luminex® xMAP®)
shipped in
wet ice
storage temp.
2-8°C
General description
The MILLIPLEX® Non-Human Primate Cytokine/Chemokine/Growth Factor Panel A 48-Plex Premixed Magnetic Bead Panel is a 48-plex kit to be used for the simultaneous quantification of the following analytes in serum or plasma samples and tissue/cell lysate and culture supernatant samples: BCA-1, sCD137, sCD40L, Eotaxin, sFASL, FGF-2, Fractalkine, G-CSF, GM-CSF, Granzyme A, Granzyme B, IFNα2, IFNγ, IL-1α,
IL-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-15, IL-16, IL-17A, IL-17E, IL-18, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10, I-TAC, MCP-1, MIG, MIP-1α, MIP-1β, MIP-3α, Perforin, RANTES, TGFα, TNFα, TNFβ, VEGF-A.
Specificity
Application
- Analytes: BCA-1, sCD137, CD40L, Eotaxin, sFASL, FGF-2, Fractalkine, G-CSF, GM-CSF, Granzyme A, Granzyme B, IFNα2, IFNγ, IL-1α, IL-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-15, IL-16, IL-17A, IL-17E, IL-18, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10, I-TAC, MCP-1, MIG, MIP-1α, MIP-1β, MIP-3α, Perforin, RANTES, TGFα, TNFα, TNFβ, VEGF-A
- Recommended Sample type: Serum, plasma or tissue/cell lysate and culture supernatant samples
- Recommended Sample dilution: Neat plasma or serum. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay.
- RANTES requires a 1:100 dilution of plasma/serum samples.
- NOTE: RANTES cannot be plexed with other cytokines in this panel due to a required 1:100 dilution of plasma/serum samples.
- Note: The serum/plasma samples of some non-human primate species may require up to 1:100 dilution in Assay Buffer for IL-8.
- Assay Run Time: Overnight or two-hour primary incubation. For best results, an overnight incubation is recommended.
- Research Category: Inflammation & Immunology
- Research Sub Category: Obesity, Metabolic Disorders, Inflammation & Autoimmune Mechanisms.
Features and Benefits
Storage and Stability
- Recommended storage for kit components is 2−8°C.
- For long-term storage, freeze reconstituted standards and controls at −20°C. Avoid multiple freeze/thaw cycles.
- DO NOT FREEZE Antibody-Immobilized Beads, Detection Antibody, and Streptavidin-Phycoerythrin.
Analysis Note
- This is an overnight or two-hour incubation assay.
- This assay requires 25 μL of neat plasma or serum (1:100 dilution for RANTES) or 25 μL cell culture supernatant per well.
- This kit may be used for the analysis of all of the Non-Human Primate Cytokine/Chemokine/Growth Factor Panel A analytes in human tissue/cell lysate and culture supernatant samples and serum or plasma samples.
- RANTES cannot be plexed with other cytokines in this panel due to a required 1:100 dilution of plasma/serum samples. For more information, please carefully review the sample preparation for serum and plasma samples and the preparation of antibody-immobilized beads sections below when assaying RANTES.
- The serum/plasma samples of some non-human primate species may require up to 1:100 dilution in Assay Buffer for IL-8.
Legal Information
Disclaimer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Target Organs
Respiratory Tract
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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