biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
11C9.10.1, monoclonal
mol wt
calculated mol wt 26.42 kDa
observed mol wt ~27 kDa
purified by
using protein G
species reactivity
human
packaging
antibody small pack of 100 μg
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
epitope sequence
Extracellular domain
Protein ID accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... FLT3LG(2323)
General description
Fms-related tyrosine kinase 3 ligand (UniProt: P49771; also known as Flt3 ligand, Flt3L, SL cytokine) is encoded by the FLT3LG gene (Gene ID: 2323) in human. Flt3 ligand is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 27-184), a transmembrane domain (aa 185-205), and a cytoplasmic domain (aa 206-235). Flt3-ligand is an important regulator of hematopoiesis that synergizes with a number of colony-stimulating factors and interleukins. It is expressed on myeloid, lymphoid, and dendritic cell progenitors. Expression of its receptor is restricted only to hematopoietic progenitors, early B cells and myeloid cells. Binding of Flt3-ligand to its receptor (Flt3) leads to receptor homodimerization and subsequent conformational changes that result in phosphorylation of its tyrosine kinase domains. This leads to rapid activation of receptor, its internalization, downstream actions, and degradation. Administration of Flt-3 ligand to mice is shown to cause a significant expansion of both CD8 + and CD8 dendritic cells at multiple sites, including spleen, lymph nodes, thymus, liver, lung, and bone marrow. Three isoforms of Flt3 have been described that are produced by alternative splicing. Isoform one is a single-pass type I membrane protein, isoform 2 is a homodimeric secreted or extracellular protein, and isoform 3 lacks the first 82 amino acids. (Ref.: Tsapogas, P., et al. (2017). Int. J. Mol. Sci. 18(6): 1115).
Specificity
Clone 11C9.10.1 is a mouse monoclonal antibody that detects Fms-related tyrosine kinase 3 ligand (Flt3L). It targets an epitope within the extracellular domain.
Immunogen
His-tagged recombinant fragment corresponding to the 158 amino acids from the N-terminal, extracellular domain of human Fms-related tyrosine kinase 3 ligand (Flt3L).
Application
Quality Control Testing
Evaluated by Western Blotting with recombinant Flt3L protein.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected human recombinant Flt3L.
Tested Applications
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Flt3L in HeLa and A431 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting with recombinant Flt3L protein.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected human recombinant Flt3L.
Tested Applications
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Flt3L in HeLa and A431 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Flt3L, clone 11C9.10.1, Cat. No. MABF3182, is mouse monoclonal antibody that detects Flt3L and is tested for use in Immunocytochemistry and Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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