MABC586
Anti-Sulf-2 Antibody, clone 8G1
clone 8G1, from mouse
Synonym(s):
Extracellular sulfatase Sulf-2, hSulf-2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
8G1, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SULF2(55959)
General description
Sulf-2 is an endoplasmic reticulum enzyme that is involved in sulfur metabolism which is important in bone, cartilage, and skeletal development. Sulf-2 is also important in neurological situations including myelination and glial cell differentiation and GDNF activity. Sulf-2 activity is also found in the kidney and important in glomerular basement membrane development and glomerular function. Finally, Sulf-2 is involved in heparan sulfate metabolism. Sulf-2 removes sulfate moieties on heparan sulfate proteoglycans which in turn modulate the heparin binding growth factor signaling activity of the cell. Sulf-2 also impacts extracellular matrix interactions and it is important during development and growth, as well as Wnt signaling and VEGF production by endothelial cells during vascularization. In disease, Sulf-2 expression in cancer is upregulated and inhibition of Sulf-2 limits cancer progression and showed increased apoptosis within tumors, thus Sulf-2 activation is being examined as a potential anti-cancer therapy.
Immunogen
Recombinant protein corresponding to Human Sulf-2.
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Tumor Markers
Tumor Markers
This Anti-Sulf-2 Antibody, clone 8G1 is validated for use in Western Blotting and Immunocytochemistry for the detection of Sulf-2.
Western Blot Analysis: 4 µg/mL of a representative lot of this antibody detected Sulf-2 in Sulf-2 transfected HEK293 cell lysates.
Immunocytochemistry Analysis: 4 µg/mL of a representative lot of this antibody detected Sulf-2 in Sulf-2 transfected HEK293 cells, but not in HEK293 cells transfected with an empty vector or Sulf-1.
Immunocytochemistry Analysis: 4 µg/mL of a representative lot of this antibody detected Sulf-2 in Sulf-2 transfected HEK293 cells, but not in HEK293 cells transfected with an empty vector or Sulf-1.
Quality
Evaluated by Western Blotting in MCF-7 conditioned media.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Sulf-2 in 10 µg of MCF-7 conditioned media.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Sulf-2 in 10 µg of MCF-7 conditioned media.
Target description
~125 & 75 kDa observed.
Sulf-2 is synthesized as a ~125 kDa pre-protein and is subsequently cleaved into ~75 kDa and ~50 kDa fragments, which are held together by disulfide bonds in the mature protein.
Sulf-2 is synthesized as a ~125 kDa pre-protein and is subsequently cleaved into ~75 kDa and ~50 kDa fragments, which are held together by disulfide bonds in the mature protein.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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