MAB1645

Anti-Dystrophin Antibody, clone 1808

clone 1808, Chemicon^®, from mouse

• Synonym(s): Anti-BMD, Anti-CMD3B, Anti-DXS142, Anti-DXS164, Anti-DXS206, Anti-DXS230, Anti-DXS239, Anti-DXS268, Anti-DXS269, Anti-DXS270, Anti-DXS272, Anti-MRX85
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1808, monoclonal
• species reactivity: chicken, mouse, rat, human
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_000109.2; NM_004006.1; NM_004007.1; NM_004009.2; NM_004010.2; NM_004011.2; NM_004012.2; NM_004013.1; NM_004014.1; NM_004015.1; NM_004016.1; NM_004017.1; NM_004018.1; NM_004019.2; NM_004020.1; NM_004021.1; NM_004022.1; NM_004023.1
• UniProt accession no.: P11532
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... DMD(1756)

Description

Specificity

By Western blot MAB1645 recognizes a single protein having a relative molecular weight of 300 kD. Reacts to mammalian skeletal muscle dystrophin. Shows no staining of mdx mouse muscle. Labels normal human muscle but not Duchenne muscle.

STAINING PATTERN:

On cryostat sections of normal mouse diaphragm MAB1645 gives strong immunofluorescence staining of the entire sarcolemma, with particularly strong staining of neuromuscular junctions.

SPECIES REACTIVITY:

Labels skeletal muscle from rat, chicken and Xenopus laevis. Labels cardiac muscle from rat and normal, but not mdx, mouse. Also labels smooth muscle from chicken gizzard.

Immunogen

Peripheral membrane proteins extracted from Torpedo postsynaptic membranes.

Application

Anti-Dystrophin Antibody, clone 1808 detects level of Dystrophin & has been published & validated for use in WB, IH.

Western blot

Immunohistochemistry

Optimal working dilutions must be determined by the end user.

Physical form

Format: Purified

Liquid in 0.02 M phosphate buffer (pH 7.6), 250 mM NaCl with 0.1% sodium azide.

Storage and Stability

Maintain at -20ºC for up to 12 months in convenient aliquots. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable