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FCMAB182F

Sigma-Aldrich

Milli-Mark® Anti-CD15-FITC Antibody, clone C3D-1

clone C3D-1, Milli-Mark®, from mouse

Synonym(s):

Alpha-(1,3)-fucosyltransferase, Galactoside 3-L-fucosyltransferase, Fucosyltransferase 4, Fucosyltransferase IV, FucT-IV, Fuc-TIV, ELAM-1 ligand fucosyltransferase

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.44

biological source

mouse

Quality Level

100

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

C3D-1, monoclonal

species reactivity

human

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgMκ

NCBI accession no.

NP_002024.1

UniProt accession no.

P22083

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... FUT4(2526)

General description

CD15 is a carbohydrate antigen carried by both glycoproteins and glycoliplds. The structure of the antigen was determined to be Galb1→4[Fuca1→3]GIcNACb1→3Galb1→R. CD15 serves as a ligand for selectins and might also be involved in cell adhesion through a direct Lewis X-Lewis X interaction. CD15 is expressed mainly on mature granulocytes and monocytes but also on immature bone marrow cells (Kannagi, 1997).
Anti-CD15, C3D-1, was included in the Sixth International Workshop and Conference on Human Leucocyte Differentiation Antigens, and studies by a number of laboratories confirmed its reactivity with CD15 (Kannagi, 1997).

Specificity

Antibody recognizes Human CD15 antigen.

Immunogen

Purified human neutrophils

Application

This Milli-Mark Anti-CD15-FITC Antibody, clone C3D-1 is validated for use in FC for the detection of CD15.

Quality

Evaluated by flow cytometry using human PBMCs

Physical form

Activated Sepharose
Purified Mouse monoclonal IgM conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Marlieke L M Jongsma et al.
Immunity, 54(1), 132-150 (2020-12-04)
HLA class I (HLA-I) glycoproteins drive immune responses by presenting antigens to cognate CD8+ T cells. This process is often hijacked by tumors and pathogens for immune evasion. Because options for restoring HLA-I antigen presentation are limited, we aimed to identify

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