BI0166
Activator Reagent 0.3M BMT in AcN
Biosynthesis Novabiochem®
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About This Item
UNSPSC Code:
41116006
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Quality Level
product line
Novabiochem®
form
liquid
reaction suitability
reaction type: Coupling Reactions
reagent type: catalyst
transition temp
flash point 2 °C (Acetonitrile)
shipped in
ambient
storage temp.
2-30°C
Related Categories
General description
Activator Reagent 0.3M 5-benzylmercaptotetrazole (BMT) in acetonitrile (AcN) is also known as benzylthiotetrazole (BTT) or benzyl-1H-tetrazole). It is used as a phosphoramidite activator reagent for oligonucleotide synthesis.
Application
0.3M BMT in AcN can be used as a reagent in solid phase phosphorylation of the oligomers. The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the 5′hydroxyl group of the growing oligonucleotide chain. The high activation potential of this reagent may be due to its greater acidity (pKa=4.08), which makes it easier to protonate the trivalent phosphorus in the initial stage of activation and increase the speed of the coupling reaction.
Features and Benefits
- efficient and rapid activation reagent that produces less
- phosphoramidite waste
- higher solubility at lower temperatures
- does not crystallize or clog lines
- safer reagent to use
Analysis Note
Color (APHA): 10APHA max
Component: Acetonitrile Purity (By GC): 99.9% min
Form: Clear Liquid
Identity (IR-spectrum): Conforms
Molarity of 5-Benzylmercaptotetrazole: 0.28-0.32M
Water (H2O): 1-30ppm
Component: Acetonitrile Purity (By GC): 99.9% min
Form: Clear Liquid
Identity (IR-spectrum): Conforms
Molarity of 5-Benzylmercaptotetrazole: 0.28-0.32M
Water (H2O): 1-30ppm
Legal Information
Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2
Storage Class Code
3 - Flammable liquids
WGK
WGK 3
Flash Point(F)
35.6 °F - (Acetonitrile)
Flash Point(C)
2 °C - (Acetonitrile)
Certificates of Analysis (COA)
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