ABT1487
Anti-alpha-Actin Antibody, Smooth Muscle
from rabbit, purified by affinity chromatography
Synonym(s):
Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein
Sign Into View Organizational & Contract Pricing
All Photos(7)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse, human
species reactivity (predicted by homology)
chicken (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ACTA1(58)
General description
Actin, aortic smooth muscle (UniProt P62736; also known as Alpha-actin-2, Cell growth-inhibiting gene 46 protein) is encoded by the ACTA2 (also known as AAT6, ACTSA, ACTVS, GIG46, MYMY5) gene (Gene ID 59) in human. Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin is encoded by the ACTA1 gene, smooth muscle alpha-actin by the ACTA2 gene, cytoplasmic beta-actin by the ACTB gene, cardiac muscle alpha-actin by the ACTC1 gene, cytoplasmic gamma-actin by the ACTG1 gene, and smooth muscle gamma-actin by the ACTG2 (a.k.a. ACTA3) gene. Although actins show >90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins, are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt.
Specificity
This polyclonal antibody targets the N-terminal sequence specific to smooth muscle alpha-actin (ACTA2), but not skeletal muscle alpha-actin (ACTA1), cytoplasmic beta-actin (ACTB), cardiac muscle alpha-actin (ACTC1), cytoplasmic gamma-actin (ACTG1), or smooth muscle gamma-actin (ACTG2; ACTA3).
Immunogen
Epitope: N-terminus.
KLH-conjugated linear peptide corresponding to the N-terminual sequence of human smooth muscle alpha-actin.
Application
Anti-alpha-Actin Antibody, Smooth Muscle is an antibody against alpha-Actin for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation.
Immunocytochemistry Analysis: 5.0 µg/mL from a representative lot immunostained smooth muscle alpha-Actin in NIH/3T3 fibroblasts, HUVECs, A431 and HeLa cells.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot immunostained smooth muscle cells in human uterus tissue sections.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated smooth muscle alpha-Actin from 500 µg of C2C12 mouse myoblast lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot immunostained smooth muscle cells in human uterus tissue sections.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated smooth muscle alpha-Actin from 500 µg of C2C12 mouse myoblast lysate.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Quality
Evaluated by Western Blotting in C2C12 mouse myoblast lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected smooth muscle alpha-Actin in 10 µg of C2C12 mouse myoblast lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected smooth muscle alpha-Actin in 10 µg of C2C12 mouse myoblast lysate.
Target description
~42 kDa observed.
Physical form
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Zaki Hakami et al.
Anatomical record (Hoboken, N.J. : 2007), 301(11), 1820-1833 (2018-10-06)
Although minor salivary glands play a significant functional role in the oral cavity, their developmental morphology and cell differentiation has been scarcely studied. This study aimed to describe the development of rat palatine glands with regard to the ultrastructural morphology
Christopher L Robinson et al.
Nature communications, 9(1), 2090-2090 (2018-05-31)
Evidence of male-to-female sexual transmission of Zika virus (ZIKV) and viral RNA in semen and sperm months after infection supports a potential role for testicular cells in ZIKV propagation. Here, we demonstrate that germ cells (GCs) are most susceptible to
Virender Kumar et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 26(12), 2798-2811 (2018-10-06)
Trans-differentiation of quiescent hepatic stellate cells (HSCs) into active myofibroblasts secretes excess amounts of extracellular matrix (ECM) proteins. miR-29b1 has the potential to treat liver fibrosis, because it targets several profibrotic genes. We previously demonstrated that miR-29b1 and the hedgehog
David Carr et al.
Breast cancer research : BCR, 24(1), 40-40 (2022-06-11)
The Bcl-3 protein is an atypical member of the inhibitor of -κB family that has dual roles as a transcriptional repressor and a coactivator for dimers of NF-κB p50 and p52. Bcl-3 is expressed in mammary adenocarcinomas and can promote
Brandan Walters et al.
Cells, 10(11) (2021-11-28)
Adipose-derived stem cells (ASCs) are an abundant and easily accessible multipotent stem cell source with potential application in smooth muscle regeneration strategies. In 3D collagen hydrogels, we investigated whether sustained release of growth factors (GF) PDGF-AB and TGF-β1 from GF-loaded
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service