AB5585
Anti-Recoverin Antibody
serum, Chemicon®
Synonym(s):
Recoverin
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse, rat, frog, chicken
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... RCVRN(5957)
mouse ... Rcvrn(19674)
General description
Recoverin is a calcium-binding protein expressed in retinal photoreceptors and midget cone bipolar cells. It appears to delay the termination of the phototransduction cascade by blocking the phosphorylation of photoexcited rhodopsin.
Specificity
Other species have not been tested.
Recognizes recoverin.
Immunogen
Recombinant human recoverin.
Application
Detect Recoverin using this Anti-Recoverin Antibody validated for use in IC, IH, IH(P) & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Sensory & PNS
Sensory & PNS
Western Blot Analysis:
1:1,000-1:5,000 dilution using ECL.
Immunohistochemistry:
1:1,000 (2 hours at room temperature or overnight at 2-8ºC) on frozen and paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde or 3% paraformaldehyde with 0.1% glutaraldehyde. Suggested permeabilization solution is 0.1% Triton X-100. Suggested blocking buffer is 0.05 M Tris saline, pH 7.4 containing 2.5% BSA, 0.1% triton and 0.05% sodium azide.
Optimal working dilutions must be determined by the end user.
1:1,000-1:5,000 dilution using ECL.
Immunohistochemistry:
1:1,000 (2 hours at room temperature or overnight at 2-8ºC) on frozen and paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde or 3% paraformaldehyde with 0.1% glutaraldehyde. Suggested permeabilization solution is 0.1% Triton X-100. Suggested blocking buffer is 0.05 M Tris saline, pH 7.4 containing 2.5% BSA, 0.1% triton and 0.05% sodium azide.
Optimal working dilutions must be determined by the end user.
Target description
26 kDa
Linkage
Replaces: AB5431P
Physical form
Rabbit polyclonal serum. Contains no preservative.
Unpurified
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
POSITIVE CONTROL: Recoverin is found in photoreceptors and midget cone bipolar cells in retina and pinealocytes in pineal gland.
POSITIVE CONTROL: Recoverin is found in photoreceptors and midget cone bipolar cells in retina and pinealocytes in pineal gland.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Genetic control of circuit function: Vsx1 and Irx5 transcription factors regulate contrast adaptation in the mouse retina.
Kerschensteiner, D; Liu, H; Cheng, CW; Demas, J; Cheng, SH; Hui, CC; Chow, RL; Wong, RO
The Journal of Neuroscience null
N Zabouri et al.
Neuroscience, 195, 145-165 (2011-08-27)
The endocannabinoid (eCB) system is thought to participate in developmental processes in the CNS. The rodent retina represents a valuable model to study CNS development because it contains well-identified cell types with established developmental timelines. The distribution of cannabinoid receptor
Adult donor rod photoreceptors integrate into the mature mouse retina.
Gust, J; Reh, TA
Investigative Ophthalmology & Visual Science null
Sonia Biswas et al.
PloS one, 9(6), e99075-e99075 (2014-06-12)
In the widely used mouse model of retinal degeneration, rd1, the loss of photoreceptors leads to rhythmic electrical activity of around 10-16 Hz in the remaining retinal network. Recent studies suggest that this oscillation is formed within the electrically coupled
J Cui et al.
Neuroscience, 224, 63-69 (2012-08-23)
Retinal bipolar cells and ganglion cells are known to possess voltage-gated T-type Ca(2+) channels. Previous electrophysiological recording studies suggested that there is differential expression of different T-type Ca(2+) channel α1 subunits among bipolar cells. The detailed expression patterns of the
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