324628

EGTA/AM

Membrane-permeable form of the Ca2+-chelating agent EGTA.

• Synonym(s): EGTA/AM, Ethyleneglycol- bis(β-aminoethyl)-N,N,Nʹ,Nʹ-tetraacetoxymethyl Ester, Ethyleneglycol-bis(β-aminoethyl)-N,N,Nʹ,Nʹ-tetraacetoxymethyl Ester
• Empirical Formula (Hill Notation): C₂₆H₄₀N₂O₁₈
• CAS Number: 99590-86-0
• Molecular Weight: 668.60
• UNSPSC Code: 12352108

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: oil
• reaction suitability: reagent type: chelator
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: light yellow
• solubility: DMSO: 20 mg/mL; DMF: soluble
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C26H40N2O18/c1-19(29)39-15-43-23(33)11-27(12-24(34)44-16-40-20(2)30)5-7-37-9-10-38-8-6-28(13-25(35)45-17-41-21(3)31)14-26(36)46-18-42-22(4)32/h5-18H2,1-4H3
• InChI key: GNJXVFXIDHCCKR-UHFFFAOYSA-N

Description

General description

Membrane-permeable form of the Ca²⁺-chelating agent EGTA.

Membrane-permeable version of the Ca²⁺-chelating agent EGTA (Cat. No. 324625). Induces apoptosis in pheochromocytoma (PC12) cells.

Application

• Arresting calcium-regulated sperm metabolic dynamics enables prolonged fertility in poultry liquid semen storage.: Researchers explored the effects of calcium regulation on sperm metabolism, demonstrating that EGTA/AM can arrest calcium dynamics, significantly enhancing the storage and fertility of poultry semen. This finding could have broad implications for agricultural practices and poultry breeding programs (Sushadi et al., 2023).

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Garcia, J.G., et al. 1992. Blood79, 2056.
Kuriyama, S., et al. 1992. Brain Res.579, 227.
Maki, A., et al. 1992. FASEB J.6, 919.
Negre-Salvayre, A., and Salvayre, R. 1992. Biochim. Biophys. Acta 1123, 207.
Ichinose, M., et al. 1992. Biochem. Biophys. Res. Commun.182, 372.
Hong, M., and Moody, T.W. 1991. Peptides12, 1315.
Schnellmann, R.G. 1991. J. Biochem. Toxicol.6, 299.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable