204882

Complement C2, Human

Complement C2, Human, is a native, single chain glycoprotein present. C2 is cleaved by the activated C1s into C2a, the 63 kDa C-terminal fragment, and C2b, the 30 kDa N-terminal fragment.

• UNSPSC Code: 12352202

Properties

• Quality Level: 100
• Assay: ≥90% (SDS-PAGE)
• form: liquid
• specific activity: ≥4×10⁵ C2H50 units/mg (>=70% of the C2 activity in normal human serum on a mg/mg basis)
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• impurities: C1, C3, C4, C5, IgG, IgA, IgM, albumin, Factor B, or hemopexin, trace
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Native, human C2 complement component. Single chain glycoprotein present in normal human serum at concentration of 20 µg/ml. Classical pathway activation results in assembly of the C1,C4b complex on target surfaces. Subsequent to binding of the C4b component to this complex, C2 is cleaved by the activated C1s subcomponent of C1 into C2a, the 63 kDa C-terminal fragment, and C2b, the 30 kDa N-terminal fragment. The smaller C2b fragment is released while the larger C2a fragment remains associated with the C1,C4b complex to form the C4b,C2a classical pathway C3 convertase enzyme. C2 concentration is based on functional activity measurements relative to a normal human serum standard pool.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, pH 6.0.

Preparation Note

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Attwood, J.T., et al. 2001. Acta Paediatr.90, 99.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable