1.51913

Purospher^® STAR NH2 (5 µm)

L × I.D. 250 mm x 4.6 mm Hibar^® RT HPLC column

• Synonym(s): Purospher^® STAR NH2 (5µm) Hibar^® RT 250-4.6
• UNSPSC Code: 41115700

Properties

• material: stainless steel column
• Agency: suitable for USP L8
• product line: Purospher^®
• feature: endcapped: no
• packaging: 1 ea of
• extent of labeling: 3.5% Carbon loading
• parameter: 400 bar pressure; 65 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable; mass spectrometry (MS): suitable (Low - Medium)
• column L × I.D.: 25 cm × 4.6 mm
• surface area: 330 m²/g
• matrix: fully porous particle
• matrix active group: NH₂ bonding phase
• particle size: 5 μm
• pore size: 120 Å pore size
• operating pH range: 1.5-10.5
• separation technique: hydrophilic interaction (HILIC); normal phase; reversed phase; supercritical fluid (SFC)
• storage temp.: no temp limit

Description

General description

Purospher^® STAR NH2, 5 µm HPLC columns are widely used for carbohydrate analysis, with a typical mobile phase consisting of acetonitrile and water. In terms of their polarity, these columns lie between bare silica and reversed-phase silica. Hence, Purospher^® STAR NH2 can also be used as an ion-exchanger. In acidic solutions, the NH2-groups are protonated (-NH3 +X-) and therefore display the characteristics of a weak anion exchanger. Medium polarity Purospher^® STAR NH2 columns possess hydrophilic as well as hydrophobic properties, thus can be used under both reversed-phase (HILIC) and normal-phase conditions. However, retention is weaker than on silica or RP-supports. Purospher^® STAR NH2 columns are based on ultra-pure fully porous silica particles (Type B). Purospher^® STAR NH2, 5 µm Hibar^® RT 250-4.6 is a ready to use stainless steel column suitable for connection to any HPLC system.The recommended guard column is Purospher^® STAR NH2 (5 µm) LiChroCART^® 4-4 part number 1.50267.0001 [https://www.sigmaaldrich.com/US/en/product/mm/150267]. For direct connection to a Hibar^® RT columns the Precolumn holder for 4-4 LiChroCART^® cartridges, part number 1.51487.0001 can be used. The column dimension of 250 mm length x 4.6 mm I.D. is the optimal choice for the HPLC separation of complex.Learn more about Purospher^® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended for the separation of alcohols, carboxylic acids, fat-soluble vitamins, nucleotides, steroids, sugars, sugar alcohols, sweeteners, and water-soluble vitamins.

Features and Benefits

• Reproducible results from run-to-run and batch-to-batch

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany

LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany

Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany