813071
TSKgel® Amide-80 HPLC Column
phase carbamoyl, L × I.D. 25 cm × 4.6 mm, 5 μm particle size
Synonym(s):
TSKgel® Amide-80, 25 cm x 4.6 mm ID, 5 μm
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
material
stainless steel
Agency
suitable for USP L68
product line
TSKgel®
manufacturer/tradename
Tosoh 13071
parameter
0-100% organic solvent
10-80 °C temperature
150 bar max. pressure
technique(s)
HPLC: suitable
L × I.D.
25 cm × 4.6 mm
surface area
450 m2/g
matrix
silica particle platform
matrix active group
carbamoyl phase
particle size
5 μm
pore size
100 Å
operating pH
2.0-7.5
separation technique
hydrophilic interaction (HILIC)
General description
TSKgel Amide-80 columns are packed with 3, 5, or 10 micron spherical silica particles that are covalently bonded with carbamoyl groups. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography. Target applications include the analysis of saccharides, glycans, oligosaccarides, peptides and polar compounds from natural products. Unique advantages of Amide-80 phase for saccharide analysis include novel hydrogen bonding retention mechanism between hydroxyl groups of the sample and the carbamoyl group in the stationary phase. The stationary phase does not react with reducing sugars and can be used at elevated temperatures (4 to 80 °C) to prevent peak splitting of carbohydrates that can occur at lower temperatures. In addition, the new, high efficiency, 3 micron columns offer shorter analysis time and enhanced sensitivity for LC-MS applications.
Physical form
Shipped in 85% acetonitrile - 15% water.
Legal Information
TSKgel is a registered trademark of Tosoh Corporation
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