ZRB1129

Anti-ZEB1 Antibody, clone 5C20 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 5C20, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 124.07 kDa; observed mol wt ~150 kDa
• purified by: using Protein A
• species reactivity: mouse, human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): ELISA: suitable; affinity binding assay: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_110378.3
• UniProt accession no.: P37275
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 5C20 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Zinc finger E-box-binding homeobox 1 (ZEB1). It targets an epitope within 22 amino acids from the C-terminal half. It is expected to react with all isoforms.

Immunogen

KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal half of human Zinc finger E-box-binding homeobox 1 (ZEB1).

Application

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ZEB1 in Jurkat cell lysate.

Tested applications

ELISA Analysis: 300 μg/mL from a representative lot detected ZEB1 in ZEB1 in an ELISA.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected ZEB1 in HeLa, NIH3T3, HEPG2, A431, and Jurkat cells.

Affinity Binding Assay: A representative lot of this antibody bound ZEB1 with a KD of 2.8 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-ZEB1, clone 5C20 ZooMAb^®, Cat. No. ZRB1129, is a recombinant Rabbit monoclonal antibody that detects ZEB1 and is tested for use in Affinity Binding Assay, ELISA, Immunocytochemistry, and Western Blotting.

Target description

Zinc finger E-box-binding homeobox 1 (UniProt: P37275; also known as NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8) is encoded by the ZEB1 (also known as AREB6, TCF8) gene (Gene ID: 6935) in human. ZEB1 is a transcription factor that is characterized by the presence of two C2H2-type flanking zinc finger clusters, which are responsible for interaction with paired CACCT(G) E-box-like promoter elements on DNA, and a centrally located POU-like homeodomain, not binding DNA. It is a prime element of a network of transcription factors controlling EMT and has been identified as an important molecule in the regulation of DNA damage, cancer cell differentiation, and metastasis. ZEB1 expression is associated with aggressive behavior, metastasis, treatment resistance and poor prognosis in different tumor types, including breast, pancreatic and lung cancer. In breast cancer, highest ZEB1 expression is observed in the aggressive triple negative and basal types. ZEB1 acts as a transcriptional repressor and inhibits interleukin-2 gene expression. It can enhance or repress the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.(Ref.: Drapela, S., et al., (2020). Front. Mol. Biosci. 7; 36).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb^® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable